摘要
根据GenBank中柑桔黄龙病菌的外膜蛋白(OMP)基因序列,设计了一套环介导等温扩增引物,建立了柑桔黄龙病的环介导等温扩增检测方法。该方法灵敏度可达25pg/μL,与实时荧光PCR方法灵敏度相同。全部反应只需一个可控温的水浴锅在60分钟内即可完成,通过肉眼观察浊度状况即可判定结果。该方法具有简便、快速、灵敏、特异等特点,适用于基层工作站的柑桔黄龙病鉴定工作。
Based on the conserved sequences of the outer membrane protein（OMP） gene of Candidatus Liberobacter asiaticus from GenBank,a set of the loop-mediated isothermal amplification（LAMP） primers was designed to amplify the target genes.The LAMP assay was established to detect HLB.The results showed that the detection limit of HLB-LAMP was 25 pg/μL,same as the real-time PCR method.The amplification could be conducted with a water bath within 60mins.The result could be visualized by naked eyes.This HLB-LAMP method is simple,rapid,sensitive,specific and suitable for primary workstation to detect HLB.
出处
《中国南方果树》
北大核心
2013年第1期8-11,共4页
South China Fruits
基金
国家质检总局科研项目(2011IK186)资助
关键词
柑桔黄龙病
环介导等温扩增
检测
Huanglongbing
loop-mediated isothermal amplification
detection
