摘要
从某生产2,4-滴丁酯的农药厂排污口采集污泥样品,驯化分离得到16株能够以2,4-滴丁酯为唯一碳源和能源生长的细菌,其中1个菌株T1对2,4-滴丁酯的抗性最强,可生长的最高浓度达到5 000mg/L。根据其形态特征、生理生化特性、16S rRNA序列分析,初步鉴定T1为巨大芽孢杆菌(Bacillus megaterium)。
Using the high pressure enrichment method, 16 bacteria strains which could utilize 2,4-D butyl ester as sole carbon source were screened and isolated from soil of sewage discharge outlet at 2,4-D butyl ester insecticide factory. One of these strains T1 could endure 5000mg/L 2,4-D butyl ester. Based on its morphological ,physiological and biochemical properties as well as the 16S rRNA sequence analysis result, T1 was tentatively identified as Bacillus megaterium.
出处
《作物杂志》
CAS
CSCD
北大核心
2013年第1期117-120,共4页
Crops
基金
国家自然科学基金项目(30971843)
国家转基因生物新品种培育重大专项(2008ZX08004-001)
黑龙江省青年基金(QC2010123)
关键词
2
4-滴丁酯
抗性
巨大芽孢杆菌
鉴定
2,4-D butyl ester
Resistant
Bacillus megaterium
Identification