可溶性Endoglin与重度子痫前期的关系

Relationship between soluble Endoglin and severe preeclampsia

目的:通过酶联法检测重度子痫前期患者和正常妊娠妇女外周血可溶性Endoglin(sEng)的表达水平,探讨其变化与重度子痫前期发病的关系。方法:选择49例重度子痫前期患者,42例无合并症孕妇,分别抽取孕妇产前,产后外周静脉血各5 ml,脐血5 ml,采用酶联免疫吸附双抗体法测定血清中sEng含量,并进行统计学分析。结果:重度子痫前期组产前、产后血清sEng水平分别为27.57(18.43～49.64)ng/L和12.20(8.57～28.57)ng/L,正常对照组sEng水平分别为14.33(8.69～18.63)ng/L和8.21(5.59～9.99)ng/L,两组比较差异均有统计学意义(P<0.001);两组脐血sEng水平分别为3.41(2.87～4.12)ng/L和(3.03±0.53)ng/L,差异有统计学意义(P<0.05);早发型重度子痫前期组产前、产后及脐血sEng水平分别为(72.55±53.92)ng/L、29.34(9.89～40.63)ng/L和3.89(3.41～4.45)ng/L,晚发型重度子痫前期组产前、产后和脐血sEng水平分别为23.94(15.84～40.75)ng/L、11.56(8.19～16.42)ng/L和(3.14±0.86)ng/L,两组比较差异均有统计学意义(P<0.05),重度子痫前期合并胎儿宫内生长受限(FGR)者产前sEng水平42.77(27.45～81.56)ng/L,明显高于无FGR者24.16(16.96～44.82)ng/L,差异有统计学意义(P<0.05)。结论:血清中sEng水平升高与重度子痫前期发病有关,尤其在早发型重度子痫前期和重度子痫前期合并FGR孕妇中血清sEng升高更显著,sEng在重度子痫前期发病中具有重要的作用。

Objective：To detect the expression levels of soluble Endoglin （sEng） in peripheral blood samples of patients with severe preeclampsia and normal pregnant women by ELISA, and explore the relationship between soluble sEng and occurrence of severe preeclamp- sia. Methods ： Forty - nine patients with severe preeclampsia and 42 pregnant women without complications were selected, venous blood sam- ples （5 ml） and mnbilical cord blood samples （5 ml） before and after delivery were obtained in pregnant women in the two groups; ELISA was used to detect serum contents of sEng in the two groups, then statistical analysis was performed. Results： The serum levels of sEng before and after delivery in severe preeclampsia group were 27.57 ng/L [ （18.43 -49. 64） rig/El and 12. 20 ng/L [ （8.57 -28.57） rig/El, re- spectively; the serum levels of sEng before and after deliver）, in normal control group were 14. 33 ng/L [ （8.69 - 18.63 ） ng/L] and 8.21 ng/L [ （5.59 -9.99） ng/Ll, respectively, there were statistically significant differences between the two groups （P 〈 0. 001 ） ; the levels of sEng in mnbilical cord blood in the two groups were 3.41 ng/L [ （2. 87 -4. 12） ng/L] and （3.03 ±0. 53） ng/L, respectively, there was statistically significant difference between the two groups （ P 〈 0. 05 ） . The serum levels of sEng before and after delivery and sEng level in umbilical cord blood in early onset severe preeclampsia group were （72. 55 ±53.92） rig/L, 29.34 ng/L [ （9.89 -40. 63） ng/L], and 3.89 ng/L [ （3.41 -4.45） ng/L] , respectively; the serum levels of sEng before and after delivery and sEng level in umbilical cord blood in late onset severe preeclampsia group were 23.94 ng/L [ （ 15.84 -40. 75） ng/L], 11.56 ng/L [ （8. 19 - 16. 42） ng/L], and （3. 14±0. 86） rig/L, respectively, there were statistically significant differences between the two groups （P 〈0. 05） ; the level of sEng before delivery in patients with severe preeclampsia combined with fetal growth restriction （FGR） was 42.77 ng/L [ （ 27.45 - 81.56） ng/L ] , which was statistically significantly higher than that in patients with simple severe preeclampsia [ 24. 16 rig/L, （16. 96 -44. 82） ng/L] （ P 〈 0. 05）. Conclusion ： The increase of serum sEng level is correlated with occurrence of severe preeclampsia, especially in pregnant women with early onset severe preeclampsia and severe preeclanlpsia combined with FGR, the increase of serum sEng level is more domi- nant, sEng plays an important role in occurence of severe preeclampsia.