摘要
目的:观察白花蛇舌草提取物(HDE)体外对人白血病细胞株K562增殖的影响,探讨HDE用于治疗白血病的作用机制。方法:以白血病K562细胞株为靶细胞,观察不同浓度的HDE对K562细胞增殖的影响,及胞内线粒体跨膜电位(ΔΨm)的水平;检测低浓度HDE处理前后胞内Sur vivin、Bcl-2基因表达。结果:HDE(6.4mL/L)能明显抑制K562细胞增殖,且与浓度呈正相关(P<0.05或P<0.01)。通过流式细胞术对碳氰化合物类亲脂性荧光染料(JC-1)检测发现,HDE能使靶细胞内ΔΨm降低,且与HDE处理浓度呈负相关;K562细胞经低浓度HDE(3.2mL/L)处理3周后,Survivin、Bcl-2基因表达均低于未经处理细胞的2.7倍和1.6倍。结论:HDE可能通过降低线粒体跨膜电位,抑制抗凋亡基因的表达,抑制K562白血病细胞增殖。
Objective： To determine the effect of hedyotis mia cell line K562 in vitro. Methods： Human leukemia herb extracts （HDE） on the proliferation of human leukecell line K562 was used and was treated by varied con- centrations of HDE（1.6,3.2,6.4,12.8,25.6ml/L）. Variation of mitochondrial transmembrane potential was measured before and after treatment. Survivin and Bcl-2 in K562 cells were detected before and ＇after treatment of 3.2ml/L HDE for 3 weeks. Results： HDE at the dose of 6.4ml/L inhibited the proliferation of K562 cells and the effect was enhanced as the dose increased. HDE decreased mitochondrial transmembrane potential in K562 cells and the decrease was negatively related to the concentration of HDE. The expression of survivin and Bcl-2 on K562 cells before the treatment of 3.2ml/L HDE was 2.7 and 1.6 times higher than those after treatment. Conclusion： HDE can inhibit the proliferation of K562 cells by decreasing mitochondrial transmembrane potential and sup- pressing anti-apoptosis genes.
出处
《浙江中西医结合杂志》
2013年第1期14-17,共4页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
