硫素对不同大豆品种异黄酮含量的影响

Effect of Sulfur on the Content of Kernal Isoflavone in Different Soybean Varieties

为探究硫素对不同大豆品种籽粒异黄酮含量的影响,寻找不同基因型大豆品种最佳施硫水平,以其提高大豆籽粒异黄酮的含量,改善其品质。选用‘黑农48’(HN48,高蛋白品种)、‘黑农37’(HN37,中间型品种)、‘黑农44’(HN44,高油品种)3个大豆品种作为试验材料。采用盆栽,每个品种设S1、S2、S3、S44个硫处理(即每千克土壤施用单质硫0g、0.02g、0.04g、0.06g)。采用紫外分光光度法测定不同大豆品种籽粒总异黄酮的含量。结果表明:同一品种不同处理大豆总异黄酮含量,HN37与HN44都表现为S2处理含量最高,HN48在S3处理下含量最高,3个品种都是适宜的硫素有利于提高总异黄酮含量;同一处理不同品种间大豆总异黄酮含量,在4个施硫条件下均表现出HN44含量最高,其次是HN37和HN48;在不同品种不同处理中,大豆总异黄酮含量峰值出现在HN44的S3水平下;施硫对3个大豆品种异黄酮含量有影响,适宜的施硫可有效提高大豆籽粒异黄酮的含量。

In this study, the effect of Sulfur （S） on the content of kernal isoflavone in different soybean varieties was analyzed. The best Sulfur levels of different genotype soybean varieties were explored, in order to increase the content of kernal isoflavone and improve their qualities. Three soybean varieties ‘（Heinong-48’, high-protein cultivar; ‘Heinong-37’, middle cultivar; and ‘Heinong-44’, high-oil cultivar） were investigated. The soybean seeds were planted in pots fertilized with S which were S1, S2, S3 and S4 （i.e., 0 g, 0.02 g, 0.04 g and 0.06 g of Sulfur） per kilogram of soil. The content of total kernal isoflavone in different soybean varieties was determined by ultraviolet spectrophotometry. The results showed that, the content of total isoflavone under the same variety of different treatment, HN37 and HN44 treatment was significantly higher than that of S2, HN48 in S3 processing under the highest content. The suitable S is known to help improving the total isoflavone content in the three varieties; same deal with the different varieties of soybean total isoflavones content, under the S1-S4 treatment HN44 was significantly highest, and then HN37 and HN48.Under different treatments in different soybean varieties, the content of total isoflavone in HN44 S3 was the highest. These findings suggested that Sulfur affected the total content of isoflavone in the three soybean varieties, and appropriate amounts of S fertilization might contribute to improving the content of kernal isoflavone.