桑树毛状根生长动力学研究

Study on the Growth Dynamics of Mulberry Hairy Roots

为建立桑树毛状根的高效培养体系,采用液体摇瓶培养法,以培养30天的毛状根湿重为指标,对桑树毛状根的培养基及培养条件进行研究。结果表明,桑树毛状根的最佳培养基是pH6.0的MS培养基,最优培养条件为26℃,130r/min,暗培养。桑树毛状根的生长表现为"慢—快—慢"的趋势,呈"S"型曲线,生长21天左右进入稳定期。

In order to establish the high efficient culture system of mulberry hairy root,the medium formula and culture conditions of mulberry hairy roots in submerged culture with shake flask were studied according to the index of the wet weight cultured 30 d.The content of total flavonoids in mulberry hairy roots was determined by spectrophotometry using rutin as standard substance.The results showed that the optimum medium was pH 6.0 MS culture medium,and the optimum culture condition was 26℃,130 r/min,dark culture.The growth trends of mulberry hairy roots was ＂ slow-fast-slow ＂,as ＂ S ＂ type curve,and the stable period was after cultured 21 days.