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AtFT基因植物表达载体的构建研究 被引量：1

Construction of Plant Expression Vector of AtFT Gene
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摘要 FT是从拟南芥克隆得到的诱导植物开花调控基因。利用FT基因表达特点,克隆拟南芥FT基因片段,重组构建了以CaMV35S为启动子、GUS为报告基因的植物表达载体pCAMBIA2301-FT与pCAMBIA2300-FT:GUS,并采用农杆菌介导法转化橡胶树体细胞胚,通过GUS组织化学染色观察橡胶树体细胞胚瞬时表达情况,结果验证了重组构建的2个载体的有效性,为进一步研究FT基因在橡胶树中的功能及应用奠定基础。 FT was an important gene involved in controlling plant flowering time from Arabidopsis thaliana.This research used FT gene expression characteristics,through cloning FT gene from Arabidopsis thaliana,plant expression vector pCAMBIA2301-FT and pCAMBIA2300-FT：GUS which had a CaMV 35S promoter and GUS reporter gene,were successfully constructed.The somatic embryos in Hevea brasiliensia were transferred by agrobacterium-mediated transformation,and the instantaneous expression of somatic embryos was observed using GUS histochemical staining.The result showed the two plants expression vectors were effective.It laid the foundation for further studying the function and application of FT genes in Hevea brasiliensia.
作者 李玉婷 华玉伟 黄天带 蔡海滨 黄华孙
机构地区 中国热带农业科学院橡胶研究所
出处 《中国农学通报》 CSCD 2013年第3期173-179,共7页 Chinese Agricultural Science Bulletin
基金 国家天然橡胶产业技术体系(nycytx-34) 国家自然科学基金"橡胶树抗白粉病新种质培育"(30960322) 中央级科研院组基本业务费"橡胶树高效遗传转化体系的建立"(1630022012006)
关键词 巴西橡胶树 FT基因 植物表达载体 体细胞胚 Hevea brasiliensia FT gene plant expression vector somatic embryo
分类号 S794.1 [农业科学—林木遗传育种]
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参考文献18

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二级参考文献1

共引文献9

同被引文献8

  • 1王玉成,张国栋,姜静.一种适用范围广的总RNA提取方法[J].植物研究,2006,26(1):84-87. 被引量：49
  • 2Kojima S., Takahashi Y., Kobayashi Y., Monna L., Sasaki T., Araki T., and Yano M., 2002, Hd3a, a rice ortholog of the A rab idopsis FT gene, promotes transition to flowering down- stream of Hdl under short-day conditions, Plant Cell Physi- ol., 43(10): 1096-1105.
  • 3Laurent C., Coral V., Seonghoe J., Fabio F., Qing Z.F., Iain S., Antonis G., Sara F., Lionel G., Colin T., and George C., 2007, FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis, Science, 316 (5827): 1030-1033.
  • 4Lin M.K., Belanger H., Lee Y.J., Varkonyi-Gasie E., Taoka K., Miura E., Xoconostle-Cazares B., Gendler K., Jorgensen R. A., Phirmey B., Lough T.J., and Lucas W.J., 2007, FLOW- ERING LOCUS T protein may act as the long-distance flori- genie signal in the cucurbits, Plant Cell, 19(5): 1488-1506.
  • 5Mitsutomo A., Yasushi K., Sumiko Y., Yasufumi D., Ayako Y., Yoko I., Harutaka I., Michitaka N., Koji G., and Takashi A., 2005, A bZIP protein mediating signals from the floral path- way integrator FT at the shoot apex., Science, 309 (5737): 1052-1056.
  • 6Shigem H., and Koji G., 2011, Arabidopsis TERMINAL FLOW- ER1 is involved in the regulation of flowering time and in- florescence development through transcriptional repression, Plant Cell, 23(5): 3172-3184.
  • 7信彩云,李志英.观赏凤梨乙烯催花机理的研究进展[J].热带农业科学,2009,29(2):78-82. 被引量：14
  • 8袁秀云,张仙云,马杰,秦喜庆.植物开花的分子调控机理研究[J].安徽农业科学,2010,38(2):614-616. 被引量：8

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中国农学通报
2013年 第3期

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