微生物生态研究中Biolog Eco微平板培养时间对分析结果的影响

Effects of Biolog Eco-plates Incubation Time on Analysis Results in Microbial Ecology Researches

Biolog Eco微平板法已广泛用于环境微生物研究,能很好地反映不同环境下微生物群落差异性特征,而分析结果可能会受Eco微平板培养时间的影响,据此,本研究从AWCD值、微生物群落多样性指数、碳源代谢强度及代谢方式和PCA分析等角度,探讨了Biolog Eco微平板培养时间对分析结果的影响特征.研究表明:Biolog Eco微平板培养时间处于AWCD曲线"拐点"前与"拐点"后对分析结果影响显著(p<0.05),且"拐点"前各培养时间段微生物群落特征的分析结果明显不同,但培养时间处于AWCD曲线"拐点"处及其后各培养时间段的分析结果基本一致,且培养时间对分析结果的影响很小或可忽略不计,可能会较客观地反映环境微生物群落特点及不同样品间的差异性.因此,采用Biolog Eco微平板分析环境微生物群落特征时,建议微平板选择240h培养并连续读数,再根据240h的AWCD曲线"拐点"进行数据合理地选取和分析,得出的结论相对可能会与实际环境情况更为接近.

The Biolog method is a useful tool in the study of microbial ecology and been widely used by the most researchers, however, Biolog Eco-plate culture time might affect the data analysis results. So the effect of Biolog Eco-plate culture time on data analysis results such as microorganism diversity index, carbon metabolic intensity, utilization rate of six carbon sources, and loadings factors of PCA （ Principal Component Analysis） were carried out in the paper. It was indicated that the Biolog Eco-plate culture time influenced data analysis results significantly （p 〈 0.05 ） and the A WCD （average well color development） value curve inflection point was an important threshold for Biolog data analysis. Microbial community characteristics were different evidently under different incubation time prior to the AWCD value curve inflection point. However, the impact of incubation time at and after the A WCD value curve inflection point on the analysis results was small or negligible and could objectively indicate the microbial community differences from different environmental samples. In conclusion, the AWCD value curve inflection point was a threshold of biolog Eco-plate culture time when investigating the environmental microbial functional diversity,furthermore, the selected data based on the AWCD value curve inflection point could objectively showed the microbial community characteristics and the discrepancy from different environmental samples.