阿替洛尔的电化学行为及与牛血清白蛋白的相互作用

Electrochemical behaviors of atenolol and its interactions with bovine serum albumin

建立了石墨烯修饰玻碳电极测定阿替洛尔的电化学分析新方法。采用电化学方法结合紫外、荧光光谱分析,研究了阿替洛尔(ATN)与牛血清白蛋白(BSA)的相互作用。实验发现,在pH 10.0的Na2CO3-NaHCO3缓冲溶液中,ATN在0.78 V处有一灵敏的氧化峰,氧化峰电流Ip与ATN的浓度在4.8～30.0μmol/L范围内呈良好的线性关系(r=0.9926,n=11),方法检出限为3μmol/L。当BSA加入ATN溶液后,ATN氧化峰电流降低,氧化峰电流的降低值ΔIp与BSA的浓度在2.6～31.0μmol/L范围内呈良好线性关系。紫外光谱表明ATN的加入使BSA的吸收峰发生蓝移。荧光光谱表明,ATN对BSA的内源荧光有显著的猝灭作用,猝灭机制为静态猝灭。

The electrochemical behaviors of atenolol （ATN） on a graphene modified glassy carbon electrode （GP/GCE） have been investigated by cyclic vohammetry am] differential pulse voltammetry. The interaction between ATN and bovine serum albumin （BSA） was investigated by linear sweep vohammetry, differential pulse voltammetry, UV absorption and fluorescence spectroscopies. The oxidation peal〈 of ATN was observed at 0.78V in pill0.0 sodium carbonate-sodium bicarbonate buffer solution. The oxidation peak current was linear with the concentration of ATN in the range of 4.8 - 30. 0 μmol/L with a detection limit of 3 μmol/L. In the presence of BSA, the oxidation current of ATN decreased and the decreasing value was linear with the BSA concentration in the range of 2.6 N 31.0 μmol/L. The binding equilibrium constant and binding ratio were calculated as 3.12 ×10^6 L/mol and 1 ： 1. The results of UV absorption spectra showed that the UV absorption peak of BSA appeared bule shift and the maximum ahsorption value increased. The results of fluorescence spectroscopy showed that the endogenous fluorescence of BSA was significantly quenched by ATN, in which the static quenching was the main mechanism of fluorescence quenching.