ISSR在油茶品种鉴别和遗传多样性分析中的应用

Identification and genetic diversity analysis of Camellia oleifera varieties using ISSR marker

分子标记技术是准确鉴别油茶品种的重要手段之一。以湖南、湖北两省主要油茶品种为试验材料,利用ISSR分子标记技术分析了66个油茶品种的遗传多样性,揭示了各品种间的亲缘关系并对其进行了有效鉴别。结果表明:从100条引物中筛选出6条引物对66个油茶品种的基因组DNA进行扩增,获得了121条可以统计的扩增产物,其中多态性条带数(NPB)为112条,多态位点百分率为92.56%(PPB),平均等位基因观察值为1.933 9,平均有效等位基因为1.650 9,基因多样性指数即平均期望杂合度为0.369 8,Shannon信息指数为0.541 7,说明油茶品种间的遗传多样性水平较高。根据扩增图谱条带的有无、多少和不同引物提供的谱带类型组合对供试油茶品种进行了鉴别。

Molecular marker is one of the important ways for precise identification of Camellia oleifera varieties.The genetic diversity of 66 varieties of C.oleifera which were from Hunan and Hubei provinces was investigated using ISSR marker to reveal the affinity of these varieties.The results showed that： 6 ISSR primers selected from 100 primers were employed to conduct PCR amplification on genome DNA of 66 varieties of cultivated C.oleifera.And a total of 121 countable bands were amplified.Among them,the number of polymorphic bands（NPB） were 112,and percentage of polymorphic bands（PPB） was 92.56%.The average value of observed number of alleles,average value of effective number of alleles,Nei＇s gene diversity and Shannon＇s information index were 1.933 9,1.650 9,0.369 8 and 0.541 7 respectively.The above parameters indicated that the level of genetic diversity in different varieties of C.oleifera was high.Different varieties of C.oleifera could be identified according to whether the polymorphic bands exist or not,the number of polymorphic bands,and the type of polymorphic bands amplified by different primers.