光肩星天牛产卵分泌物的化学成分分析

Composition analysis of the oviposition secretion of Anoplophora glabripennis

通过室内饲养产卵期光肩星天牛成虫并采集其产卵分泌物,对光肩星天牛产卵分泌物进行组分分析。利用烘干法、Bradford法、蒽酮比色法、茚三酮试剂显色法、SDS-PAGE对其组分中水、蛋白质、可溶性糖、游离氨基酸的含量及蛋白质组分进行了测定。结果表明:光肩星天牛产卵分泌物组分中含水量为81.98%,蛋白质含量为4.14%,可溶性糖及游离氨基酸的含量依次为0.51%和0.06%(均为质量分数),蛋白质组分中主要蛋白的分子质量为107.1、86.7、62.2、47.5、39.0 ku,其中107.1～86.7 ku与47.5 ku为强谱带区。

To analyze the composition of Anoplophora glabripennis oviposition secretion,A.glabripennis were kept indoors during the spawning period,and oviposition secretion of which was collected.The oven drying method,Bradford method,anthrone colorimetry,Ninhydrin reagent colorimetric method and SDS-PAGE were used to analyze and determinate the fractions of water,protein,soluble sugar and free amino acid content and protein fractions in oviposition secretion.The results showed that oviposition secretion components of A.glabripennis included water（81.98%）,protein（4.14%）,soluble sugar（0.51%） and free amino acid（0.06%）.The molecular weight of main protein components were 107.1 ku,86.7 ku,62.2 ku,47.5 ku and 39.0 ku.The strong spectral bands were 107.1 ku to 86.7 ku and 47.5 ku.