磁性壳聚糖微球制备及生物相容性的研究

Preparation and biocompatibility of magnetic chitosan microspheres

目的:制备5-氟尿嘧啶磁性壳聚糖微球并评价空载磁性壳聚糖微球的生物相容性。方法:采用乳化交联法制备5-氟尿嘧啶磁性壳聚糖微球并优化制备工艺,采用扫描电镜和振动样品磁强计(VSM)对微球进行表征;四噻唑蓝法观察细胞增殖情况,评估磁性壳聚糖微球的体外细胞毒性;溶血实验和血常规检查评估磁性壳聚糖微球的血液相容性;植埋实验评估磁性壳聚糖微球的组织相容性。结果:经过优化后的5-氟尿嘧啶磁性壳聚糖微球包封率和载药量分别为70.2%和12.3%,Z-均粒径为1 479.6 nm,饱和磁化度为4.79 emu.g-1,微球形态良好、均匀圆整;自制空载磁性壳聚糖微球体外细胞相容性符合要求,显示出良好的血液相容性和体内组织相容性。结论:优化了5-氟尿嘧啶磁性壳聚糖微球的制备工艺,制备的空载磁性壳聚糖微球具有很好的生物相容性。

Objective： To prepare 5-fluorouracil-loaded magnetic chitosan microspheres and to estimate the biocompatibility of unloaded magnetic chitosan mierospheres. Methods： 5-Fluorouracil-loaded magnetic chitosan microspheres were established by emulsification cross-linking method and the preparation procedure was optimized. The obtained magnetic mierospheres were characterized by scanning electron microscopy and VSM. MTT assay was used to evaluate the in vitro cytotoxicity test. Haemolysis test and blood routine examination were used to evaluate blood compatibility. The implantation test was used to evaluate histoeompatibility. Results： Encapsulation efficien- cy and loading efficiency of 5-fluorouracil in magnetic chitosan microspheres reached 70.2% and 12.3% after opti- mization. The Z-average diameter was 1 479.6 nm. The saturation magnetization is 4.79 emu. g-1 The micro- spheres were spherical shape with a regular surface. Compatibility of unloaded magnetic chitosan microspheres was in coincidence with the criteria. Conclusion： The optimized preparation condition is confirmed and repeated. Un- loaded magnetic chitosan microspheres has a superior biocompatibility.