急性髓性白血病细胞体外诱导脐血细胞毒性T淋巴细胞增殖及抗白血病作用的研究

Studies on the specific anti-leukemia cell effect of cytotoxic T lymphocytes induced by acute myeloid leukemia-derived dendritic cells

目的研究体外培养急性髓性白血病树突状细胞(AML-DC),并利用该细胞联合细胞因子诱导脐血产生细胞毒性T淋巴细胞(CTL),观察CTL对急性白血病细胞的杀伤效应,探讨从AML-DC体外诱导产生CTL的可行性。方法从急性髓性白血病细胞诱导AML-DC,联合细胞因子体外诱导脐血T细胞活化及增殖,流式细胞术检测培养前后的T淋巴细胞亚群变化,利用LDH试剂盒检测诱导后T细胞对相应急性白血病细胞的杀伤活性。结果可从人AML细胞中诱导出AML-DC,脐血T细胞在体外经过诱导培养后可获得增殖,T淋巴细胞比例较培养前明显增高,其中在AML-DC诱导组中,CD3+T淋巴细胞亚群比例达到(79.7±3.70)%,该T淋巴细胞对相应急性白血病细胞的杀伤效率最高达(48.35±12.75)%,与培养前及培养后无AML-DC刺激组相比,经过特异诱导培养的T细胞对相应白血病细胞杀伤作用大大加强(P<0.05)。结论 AML-DC联合细胞因子可以诱导活化脐血白血病细胞特异性细胞毒性T淋巴细胞(CTL),该细胞能对相应白血病细胞产生特异性杀伤效应。

Objective To investigate the specific anti-leukemia cell effect of cytotoxic T lymphocytes（CTL） induced by acute myeloid leukemia-derived dendritic cells （AML-DC）. Methods Acute myeloid leukemic cells were induced to differentiate into AML-DC. T cells from umbilical cord blood were activated with the cytokine combined with AML-DC. T cells were identified by flow cytometer（FCM）. The anti-tumor activity of T cells was assayed with LDH method. Results Highly reactive anti-AML T cells were generated and expanded. The CD3^＋ T cells rate was （79.7 ± 3.7 ）% in the group after stimulation by AML-DCs. These T cells caused specific lysis of （48.35 ± 12. 75 ） % target AML cells. And the difference was significant compared with T cells without stimulation of AML- DCs （P 〈 0. 05 ）. Conclusion Leukemic cells f patients with acute myeloid leukemia could be differentiated to DC-like cells possessing the ability of stimulating anti-leukemic immune response.