低温胁迫下高山离子芥消减文库的构建

Library construction of differentially expressed genes of Chorispora bungeana under cold stress

以0℃低温处理12 h的高山离子芥幼苗cDNA为检测子,以正常生长温度下的高山离子芥幼苗cDNA为驱动子,应用抑制性消减杂交技术,构建冰缘植物高山离子芥的消减文库,筛选在低温下高表达的基因片段.随机挑取96个阳性克隆进行测序分析,得到39个独立的基因片段,26个和已知基因有较高的同源性,13个为未知基因.基因分析表明:高山离子芥在低温胁迫下,微丝交联蛋白Actin cross linking protein很可能在感受低温的过程中行使作用,而DREB2A和ABF1协同作用一起调节下游冷响应基因的表达,为进一步研究高山离子芥的抗冻机制奠定了基础.

Suppression subtractive hybridization（SSH） was applied to identify genes involved in cold stress in an alpine subnival plant Chorispora bungeana,taking Chorispora bungeana seedlings under 0℃for 12 h as the tester and untreated seedlings as the driver and and the differentially expressed genes library was constructed.A total of 96 positive clones were sequenced,and 39 unigenes were identified and analyzed,with the result that 26 showed a high identity with known genes while 13 had no matches in the GeneBank and were listed as unknown genes.Gene function analysis showed that an actin cross the linking protein（F1） might play a role during the cold sensing while DREB2A and ABF1 might work together to regulate the downstream cold responsive genes. These results can lay a foundation for further study of Chorispora bungeana cold tolerance mechanisms.