茶叶中多酚氧化酶提取的响应面优化

Optimization of extraction on polyphenol oxidase from green tea via response surface methodology

优化了茶叶多酚氧化酶的提取工艺,为进一步的纯化提供了理论参数。在单因素试验的基础上,选取了提取缓冲液pH、保护剂添加量、料液比3个因素进行了CCD响应面试验设计,对提取参数进行优化。结果显示,当缓冲液pH为5.6,保护剂PVP添加量为0.16%,料液比为1:4.5时,所得的酶活最高。采用响应面分析,能得到最高的酶活提取条件,并且能较为准确的预测试验结果。

The extraction technique of polyphenol oxidase from green tea was optimized to provide theoretical parameters for further purification. On the basis of single-factor experiments, the effects of different pH value of buffer, the amount of polivinpolypyrrolidone （PVP） and ratio of buffer to material on the extraction of polyphenol oxidase were investigated with a three-factor CCD design. The extraction parameters were optimized with response surface methodology. The results indicated that polyphenol Oxidase activity increased to max as pH value rose to 5.6, the amount of PVP rose to 0.16% and the ratio of buffer to material rose to 4.5：1. Extraction model of polyphenol oxidase established by CCD method can precisely predict the experiment results. In the best conditions deduced from the mode, the extraction activity of polyphenol oxidase is high.