Apelin经Akt/AMPK信号通路促进心肌微血管内皮细胞血管生成

Apelin promotes angiogenesis of myocardial micro-vascular endothelial cells via Akt/AMPK pathway

目的:探讨apelin-13对大鼠心肌微血管内皮细胞(MVECs)的生物学效应及其可能的信号通路。方法:体外培养大鼠的心肌微血管内皮细胞。给予不同浓度apelin-13,采用MTT法观察细胞增殖能力、划痕损伤修复和Transwell方法观察细胞迁移能力、体外管腔样结构形成方法观察细胞管腔成能力。进一步采用蛋白印迹法观察apelin-13对Akt和AMPK分子蛋白的磷酸化水平的影响。结果:Apelin-13能增加大鼠微血管内皮细胞的增殖、迁移和体外管腔形成能力,且呈剂量依赖性在200 nmol/L浓度时促血管生成能力最佳。Apelin-13能上调细胞的Akt和AMPK磷酸化程度。结论:Apelin-13可促进MVECs的血管生成,可能与上调Akt和AMPK的磷酸化相关。

Objective:To investigate whether apelin-13 has angiogenesis effects in myocardial micro-vascular endothelial cells(MVECs).Methods:Rat MVECs were cultured in vitro.The cells were treated with apelin-13 at different concentrations.MTT assay was used to determine the proliferation ability.Migration ability was measured using cell scratches and a modified boyden chamber.The tube formation ability of MVECs was tested by Matrigel.The phosphorylation of AMPK and Akt which may be induced by apelin-13 was measured with Western blot.Results:Apelin enhanced the proliferation,migration and tube-like structure formation of MVECs in vitro,which was concentration dependent.Furthermore,apelin-13 signicantly up-regulated the phosphorylation levels of both Akt and AMPK.Conclusion:Apelin has angiogenesis effect in MVECs,which may be related to the upgrading of Akt/AMPK phosphorylation.