桑叶HILIC特征指纹图谱研究

Study on the Hydrophilic Interaction Chromatography Specific Fingerprint of Mulberry leaves

应用亲水色谱法(HILIC)建立了桑叶药材的指纹图谱,并对10批桑叶样品进行分析,为桑叶药材的真伪鉴别及质量控制提供了新方法。采用HILIC XBridgeTMAmide色谱柱,以乙腈-水(含0.2%甲酸、20mmol/L甲酸铵、20%甲醇)为流动相,梯度洗脱,流速为0.8 mL/min,柱温为25℃,检测波长为322 nm,进样量为20μL。该方法具有良好的精密度、重现性和稳定性,检测的10批桑叶指纹图谱有17个共有峰,4个特征峰,采用ESI-TOF/MS对4个特征峰进行了指认,结合相似度分析可以用于不同产区桑叶药材的鉴别。桑叶HILIC指纹图谱有望成为桑叶药材真伪鉴别及质量控制的有力工具。

A hydrophilic interaction chromatography（HILIC） fingerprinting method had been established for the quality evaluation and discrimination of mulberry leaves.The chromatographic fingerprints were determined by injecting 20 μL of the sample solution each time into the hydrophilic interaction chromatography column（XBridgeTM Amide column） with the gradient elution solvent system composed of water（containing 0.2% formic acid,20 mmol/L ammonium formate and 20% methanol） and acetonitrile.The flow rate was 0.8 mL/min,the column temperature was maintained at 25 ℃ and the signals were acquired at 322 nm.The result indicated that the developed HILIC method was simple,accurate and reliable for the development of mulberry leaves fingerprint.The HILIC fingerprints for ten samples tested contained the same seventeen peaks and four characteristic peaks.Combining with the similarity analysis,the method could be used to discriminate different origins of mulberry leaf samples.The HILIC fingerprinting technique is expected to become an important tool in the identification and quality evaluation of mulberry leaves.