16S rDNA基因文库技术分析发酵床细菌群落的多样性

The Bacteria Diversity of Fermentation Bed in Pig-farming by 16S rDNA Gene Clone Library Analysis

细菌群落在养殖发酵床中担负着重要的调节与废物发酵转化作用.直接从不同发酵床层次样品中提取细菌总DNA,构建发酵床不同层次垫料的细菌基因克隆文库.试验表明,发酵床表层45株细菌中未培养微生物占39.5%,剩余细菌分属13个不同种,优势菌不明显;中层发酵床垫料45株细菌中未培养微生物占15.5%,剩余细菌分属10个不同种,优势细菌为Rhodanobacter sp.和Frateuria sp.;深层发酵床垫料中检测到的44株细菌不含未培养微生物,该层细菌主要有14个不同的种,优势细菌为Rhodanobacter sp.占47.7%,其余种属相对较少且数量平均,表明发酵床垫料细菌呈明显的多样性.

Microorganism has an important regulatory and bioconversion function in fermentation bed of pig-breeding. The bacterial DNA samples are directly extracted from different depth in fermentation bed. The bacterial gene clone library is constructed correspondingly for these different types of samples. The results show that the uncultured microorganism appeared in the surface of the fermentation bed, which accounts for 39.5% of the total 45 strains detected. The others belong to 13 different species of bacteria and no predominant bacteria detected. In the middle layer of fermentation bed, uncultured microorganism accounts for 15.5% of the total 45 strains detected. The others consist of 10 different species of bacteria. The predominant bacteria are Rhodanobacter sp. and Frateuria sp. respectively. The uncultured microorganism is not detected in all 44 bacteria strains in the bottom of fermentation bed. There are 14 different bacteria genus in this layer. The predominant bacteria is Rhodanobacter sp., accounting for 47.7% in all the bacteria detected. The others are in a less proportion of total microbial population in this layer. The results demonstrate that the fermented bed in pig-farming manifested an obvious bacterial diversity.