血浆(1-3)-β-D葡聚糖定量检测对深部真菌感染早期诊断的价值

Early Diagnosis Value of Plasma(1-3)-β-D Glucan Detection for Deep Fungal Infection

目的:探讨血浆(1-3)-β-D葡聚糖定量检测对深部真菌感染早期诊断的价值。方法:采用MB-80微生物动态快速检测系统以及GKT-1MSet动态真菌检测试剂盒定量检测血浆中(1-3)-β-D葡聚糖含量,并与传统真菌培养法作比较,研究(1-3)-β-D葡聚糖定量检测在深部真菌感染早期诊断中的价值。结果:58例疑似深部真菌感染患者中,血浆(1-3)-β-D葡聚糖检测阳性为40例,阳性率为68.97%,含量为(70.25±48.88)pg/ml,阴性为18例,含量为(8.53±2.32)pg/ml。经t检验分析,阳性组含量明显高于阴性组(t=2.023,P<0.05);同时传统真菌培养法检测阳性为29例,阳性率为50.00%,血浆(1-3)-β-D葡聚糖定量检测阳性率明显高于传统真菌培养法(χ2=33.621,P<0.05)。结论:血浆(1-3)-β-D葡聚糖定量检测方法简便、快速,阳性率高,可用于深部真菌感染的早期诊断。

Objective：To investigate the value of plasma（1-3）-β-D glucan quantitative detection in early diagnosis of deep fungal infection.Methods：To study the value of serum（1-3）-β-D glucan quantitative detection in early diagnosis of deep fungal infection by quantitatively detecting the content of plasma（1-3）-β-D glucan by Rapid Kinetic Detection System of Microbiology MB-80 and Kinetic Mycetes Detection Kit GKT-1M Set,and comparing to traditional fungal culture method.Results：We detected 58 patients who are suspected to deep fungal infection.There are 40 patients with positive results of plasma（1-3）-β-D glucan quantitative detection and the positive rate is 68.97%,the content is（70.25±48.88）pg/ml while there are 18 patients with negative results and the content is（8.53±2.32）pg/ml.Analyze the data by t-test,the content of positive group is significantly higher than that of negative group（t=2.023,P0.05）;29 patients are detected positive by traditional fungal culture method and the positive rate is 50.00%,therefore,the positive rate of plasma（1-3）-β-D glucan quantitative detection is significantly higher than that of traditional fungal culture method（χ2=33.621,P0.05）.Conclusion：Plasma（1-3）-β-D glucan quantitative detection is a simple,convenient,rapid and high positive rate method to early diagnosis of deep fungal infection.