胃安宁颗粒抗胃癌转移及对多种黏附分子的影响

Influence of Weianning Granules on Anti Gastric Cancer Metastasis And a Variety of Adhesion Molecules

目的:观察胃安宁颗粒对原位移植瘤及其转移的影响,以及对胃癌组织中相关黏附分子表达的影响。方法:建立裸鼠胃癌原位移植模型,给予胃安宁颗粒,测定移植瘤形成及生长和转移情况;应用免疫组织化学和RT-PCR方法检测移植瘤组织中黏附分子E-cad、ICAM-1,CD44蛋白及mRNA的表达。结果:胃安宁颗粒能明显抑制胃癌原位癌的生长,减少转移灶;明显上调E-cad的表达水平,下调ICAM-1,CD44表达水平。结论:胃安宁颗粒对裸鼠原位胃癌的生长及其转移也有明显的抑制作用,其作用与影响相关黏附分子的表达有关。

Objective：To observe the Weianning granules of orthotopic transplantation tumor and its metastases influences,as well as on adhesion molecule expression in gastric cancer tissue and related effects.Methods：Establishment of a nude mouse gastric cancer orthotopic transplantation model,give Weianning granules,determination of the graft aneurysm formation and growth and metastasis;the application of immunohistochemistry and RT-PCR detection of transplanted tumor tissue adhesion molecules E-cad,ICAM-1,CD44 protein and mRNA expression.Results：Weianning granules can significantly inhibit gastric carcinoma in situ growth,reduce metastasis;marked upregulation of the expression level of E-cad,downregulation of ICAM-1,the expression level of CD44.Conclusion：Weianning granules on gastric carcinoma growth and metastasis in nude mice also was significantly inhibited,the role and influence of adhesion molecules expression.