高效毛细管电泳法测定夏枯草中4种活性成分的含量

Four Active Component Assay of Xiakucao with High Performance Capillary Electrophoresis

目的:建立高效毛细管电泳法测定夏枯草中4种活性成分齐墩果酸、熊果酸、迷迭香酸和咖啡酸的含量。方法:采用胶束毛细管电泳法测定齐墩果酸、熊果酸的含量;毛细管区带电泳法测定迷迭香酸和咖啡酸的含量。结果:齐墩果酸、熊果酸、迷迭香酸、咖啡酸在54.8～657.6 mg.L-1、102.4～819.2 mg.L-1、20.4～163.2 mg.L-1、10.2～81.6 mg.L-1内线性关系良好;r分别为0.999 8、0.999 9、0.999 4、0.999 2;平均回收率为95.8%、95.6%、97.7%、96.7%;RSD为1.9%、1.9%、1.6%、2.0%。结论:该方法简便、快速、准确并且专属性强,可用于测定夏枯草药材中齐墩果酸、熊果酸、迷迭香酸和咖啡酸4种活性成分的含量。

Objective：To establish a high performance capillary electrophoresis（HPCE） method to determine the content of oleanolic acid,ursolic acid,rosmarinci acid and caffeic acid in Xiakucao.Methods：To determine the content of oleanolic acid and ursolic acid using micellar capillary electric method.Determine the content of rosmarinci acid and caffeic acid using capillary zone electrophoresis.Results：The content of oleanolic acid,ursolic acid,rosmarinci acid and caffeic acid had good linear relation in 54.8-657.6 mg·L-1,102.4-819.2 mg·L-1,20.4-163.2 mg·L-1 and 10.2-81.6 mg·L-1.R was separately 0.999 8,0.999 9,0.999 4 and 0.999 2.The average recovery was 95.8%,95.6%,97.7% and 96.7%.RSD was 1.9%,1.9%,1.6% and 2.0%.Conclusion：This method was simple,rapid,accurate with a strong specificity and can be used as a determination of the content of oleanolic acid,ursolic acid,rosmarinci acid and caffeic acid in Xiakucao.