标本不同处理因素对部分生化项目的影响

Impacts of different treatments of specimen on biochemical tests of some items

目的探讨标本不同处理因素对部分生化检验项目的影响。方法血液标本立即检测设为对照组,(1)标本放置3、7、24h后检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(GGT)、乳酸脱氢酶(LDH);(2)全血保存和血清分离保存4℃24h,检测ALT、LDH、血糖(GLU);(3)密封保存和未密封保存检测ALP。结果 (1)对照组、标本放置3、7、24h各组ALT检测结果分别是(16.00±6.48)、(16.01±6.72)、(16.37±6.92)、(16.57±7.09)U/L,均P>0.05;AST(18.3±4.72)、(18.2±4.68)、(19.1±4.33)、(17.8±4.75)U/L,均P>0.05;ALP(78.1±16.28)、(77.1±15.82)、(78.2±16.55)、(77.4±16.15)U/L,均P>0.05;GGT(11.4±5.62)、(11.3±5.65)、(12.0±5.33)、(12.0±5.69)U/L,均P>0.05;LDH(183.8±21.96)、(183.3±22.74)(P>0.05)、(188.4±25.15)(P<0.01)、(196.2±26.41)U/L(P<0.01);(2)对照组、全血保存、血清分离保存各组:ALT(11.29±3.19)、(12.14±3.07)、(11.41±2.97)U/L,均P>0.05;LDH(194.83±27.09)、(217.41±28.35)(P<0.01)、(202.75±27.22)U/L(P>0.05);GLU(4.88±0.68)、(3.30±0.81)(P<0.01)、(5.08±0.80)mmol/L(P>0.05);(3)密封保存:对照组、全血保存、血清保存ALP(74.33±11.79)、(74.60±12.05)、(74.66±11.61)U/L(均P>0.05);未密封保存:对照组、全血保存、血清保存ALP(70.80±7.85)、(85.86±6.65)(P<0.01)、(92.60±5.67)U/L(P<0.01)。结论标本放置3、7、24h对ALT、AST、ALP、GGT检测无明显影响,放置7、24h对LDH检测有显著影响;全血保存24h对LDH、GLU结果存在明显差异;未密封保存对生化指标有明显影响。应注意分析前质量控制,降低分析前误差。

Objective To observe the impacts of different treatments of specimen on biochemical tests of some items.Methods The results that specimens were tested right away after collections were put into the matched group.（1） the indices of ALT,AST,ALP,GGT and LDH were determined separately 3 hours,7 hours and 24 hours after the blood were collected.（2） The indices of ALT,LDH and GLU were determined separately when the blood specimens and the serum specimens were stored at 4 ℃ for 24 hours.（3） The index of ALP was determined when the blood specimens and the serum specimens were stored in the condition of sealed and unsealed at 4 ℃ for 24 hours.Results ALT of control sample and samples laid side for 3,7 and 24 hours were 16.00±6.48,16.01±6.72,16.37±6.92,16.57±7.09（P0.05）.AST of control sample and samples layed side for 3,7 and 24 hours were 18.3±4.72,18.2±4.68,19.1±4.33,17.8±4.75（P0.05）.ALP of samples layed side for 3,7 and 24 hours were 78.1±16.28,77.1±15.82,78.2±16.55,77.4±16.15（P0.05）.GGT of control sample and samples layed side for 3,7 and 24 hours were 11.4±5.62,11.3±5.65,12.0±5.33,12.0±5.69（P0.05）.LDH of control sample and samples layed side for 3,7 and 24 hours were 183.8±21.96,183.3±22.74（P0.05）,188.4±25.15（P0.01）,196.2±26.41（P0.01）.ALT of control sample,blood specimens and the separated serum specimens were 11.29±3.19,12.14±3.07,11.41±2.97（P0.05）.LDH of control sample,blood specimens and the separated serum specimens were 194.83±27.09,217.41±28.35（P0.01）,202.75±27.22（P0.05）.GLU of control sample,blood specimens and the separated serum specimens were 4.88±0.68,3.30±0.81（P0.01）,5.08±0.80（P0.05）.For Sealed storage,ALT of control sample,blood specimens and the separated serum specimens were 74.33±11.79,74.60±12.05,74.66±11.61（P0.05）.For unsealed storage,ALT of control sample,blood specimens and the separated serum specimens were 70.80±7.85,85.86±6.65（P0.01）,92.60±5.67.Conclusion the time of laying aside,the blood being stored and the un-covered condition have the impacts on the results.