男性不育伴乙型肝炎病毒感染患者精子DNA完整性的初步研究

Sperm DNA integrity of infertile males with hepatitis B virus infection

目的:探讨男性不育伴乙型肝炎病毒感染患者精子DNA完整性。方法:应用精子染色质结构分析检测90例男性不育伴乙型肝炎病毒感染者(A组)、82例男性不育者(B组)及70例正常生育者(C组)的精子DNA完整性:DNA碎片化指数(DFI)和高DNA可染性(HDS),分别比较3组之间精子DNA完整性的差异。结果:精子DNA完整性:DFI:A组为(28.17±13.06)%,高于B组(26.64±9.79)%和C组(15.67±4.73)%,A组及B组分别与C组比较差异有统计学意义(P<0.05),A组与B组比较差异无统计学意义(P>0.05);HDS:A组为(10.83±5.60)%,高于B组(9.04±3.48)%及C组(8.04±2.25)%,A组与C组比较差异有统计学意义(P<0.05)。结论:男性不育患者的精子DNA完整性与正常生育者存在差异,若伴乙型肝炎病毒感染,会进一步加重精子DNA损伤,主要表现为精子核成熟异常。

[ Abstract] Objective： To investigate sperm DNA integrity in male infertility patients with hepatitis B virus （HBV） infection. Methods： This study included 90 infertile men with HBV infection （group A）, 82 infertile men without HBV infection （group B） and 70 normal fertile men （group C）. We detected sperm DNA integrity among the subjects, including DNA fragmentation index （DFI） and high DNA stainability （HDS）, by sperm chromatin structure assay （SCSA） , and compared them among the three groups. Re- sults ： DFI was higher in group A （ [ 28.17 ± 13.06 ] % ） than in B （ [ 26.64 ±9.79 ] % ） and C （ [ 15.67 ±4.73 ] % ）, significantly higher in A and B than in C （ P 〈 0.05 ） but with no significant difference between A and B （ P 〉 O. 05 ）. HDS was higher in group A （ [10.83± 5.60 ] % ） than in B （[ 9.04± 3.48 ] % ） and C （ [8.04 ± 2.25 ] % ）, with significant difference between A and C （ P 〈 0.05 ）. Conclusion ： Sperm DNA integrity of infertile males is significantly different from that of normal fertile men, and infertilitywith HBV infection further impairs sperm DNA, which is manifested by abnormal sperm nuclear maturity.