铕对决明发根生长及有效成分生成的作用

Effect of EuCl_3 on Hairy Root Growth of Cassia Obtusifolia and Production of Its Active Principle

以决明发根为材料 ,在培养基中附加 0 0 0 1～ 10mg·L- 1 Eu3+ (EuCl3) ,测量发根的鲜重和干重 ,用高效液相色谱法测定游离蒽醌化合物含量。发现极低剂量 ( 0 0 0 1,0 0 1,1 0mg·L- 1 )Eu3+ 抑制生长 ,0 0 0 1mg·L- 1 Eu3+ 抑制作用最强 ,干重比对照降低 2 2 % ;较低剂量( 10mg·L- 1 )Eu3+ 促进生长 ,干重比对照增加 2 7% ;0 0 0 1,0 0 1,0 1,1 0mg·L- 1 Eu3+ 各个处理的游离蒽醌化合物总含量均低于对照 ,而10mg·L- 1 Eu3+ 处理的含量高于对照 2 6% ,高于Eu3+ 1 0mg·L- 1 处理 97%。10mg·L- 1 Eu3+ 能有效增强决明发根的生长、提高游离蒽醌化合物总含量和产量。

Treated with extremely low EuCl 3(0 001, 0 01, 1 0 mg·L -1 ) in MS medium, the hairy root growth of Cassia obtusifolica was inhibited. The inhibition was strongest at the treatment with 0 001 mg·L -1 EuCl 3, and the dry weight hairy roots was 22% lower than that of control. Treated with rather low EuCl 3 (10 mg·L -1 ) in MS medium, the hairy root growth was enhanced, and the dry weight of hairy roots was 27% higher than that of control. In the each treatment with 0 001 to 1 0 mg·L -1 EuCl 3 in MS medium, the total content of six free anthraquinones was lower than that of control. While treated with 10 mg·L -1 EuCl 3, the total content of six free anthraquinones was 26% higher than that of control, 97% higher than the treatment of 1 0 mg·L -1 EuCl 3. Treatment with 10 mg·L -1 EuCl 3 in MS medium enhances the growth of hairy root of Cassia obtusifolia and improves the total content and production of six free anthraquinones in them.