镧离子及其配合物对植物花粉细胞质膜透性及花粉萌发的影响

Effects of Lanthanum Ion and Its Complexes on Permeability of Pollen Plasmalemma and Pollen Germination

以植物花粉细胞为试验体系 ,研究La3+ 及其配合物对花粉质膜透性及花粉萌发的影响。La3+ 浓度为 1× 10 - 7～ 1× 10 - 4 mol·L- 1 ,可显著减小细胞的质膜透性 ;稀土稳定配合物La3+ EDTA对细胞质膜透性基本无影响 ,而La3+ 柠檬酸、La3+ 酒石酸的配合物对质膜透性的影响与其配比有关 ,当配比不大于 1∶3时 ,明显降低质膜透性 ,达到 1∶10时 ,无任何作用。La3+ 浓度在合适的范围内 ( 2 5～ 2 0 μmol·L- 1 )对花粉萌发有明显的促进作用。因此 ,La3+ 及其某些配合物浓度在 1× 10 - 6 ～ 1× 10 - 5 mol·L- 1 范围内不仅可以减小花粉细胞的质膜透性 ,而且还能促进花粉萌发。

Effect of La 3+ and its complexes on permeability of pollen plasmalemma and pollen germination were studied. La(NO 3) 3 in the range of 1×10 -7 ～ 1×10 -4 mol·L -1 obviously inhibits the permeability of pollen plasmalemma, while La 3+ EDTA complexes have no effect on the permeability. The effects of La 3+ citrate and La 3+ tartrate complexes on permeability of pollen plasmalemma are related to the ratio of ligand to La 3+ . When the ratio is less than 3∶1, the complexes reduce the permeability of pollen plasmalemma, while the ratio is to 10∶1, and the complexes have no any effect. La 3+ in the range of 2 5～20 μmol·L -1 promotes pollen germination, but the pollen germination is inhibited as the concentration is above 80 μmol·L -1 . The above results indicate that La 3+ and some complexes species are beneficial to the reduction of permeability of pollen plasmalemma and the promotation of pollen germination when the concentration is in the range of 1×10 -6 ～1×10 -5 mol·L -1 .