单抗靶向超抗原SEA体内抗膀胱肿瘤实验研究

The anti-tumor effect of monoclonal antibody targeted superantigen SEA on human bladder cancer in vivo

背景与目的:超抗原葡萄球菌肠毒素A(staphylococcal enterotoxin A,SEA)在体内、外以及临床的膀胱灌注治疗试验中对膀胱癌均具有明显的抑瘤效应,本研究旨在探讨单抗靶向的超抗原SEA体内对人膀胱癌的抑瘤作用。方法:以腹腔注射人外周血单个核细胞、皮下注射人膀胱癌BIU87细胞建立人免疫重建荷人膀胱癌SCID小鼠复合模型,再将模型小鼠随机分为对照组、SEA组及单抗靶向SEA实验组,分别予以PBS、SEA及单抗靶向SEA瘤周注射,以SonoSite彩超在建模后6周内检测小鼠皮下移植瘤体积、瘤体血流。结果:单抗靶向SEA组瘤体体积、瘤体血流均小于对照组及SEA组,在建模后4、5、6周差异均有统计学意义(P<0.05)。结论:单抗靶向的超抗原SEA对膀胱癌皮下移植瘤生长的抑制作用更强,可能与其进一步减少瘤体血流有关。

Background and purpose： Staphylococcal enterotoxin A （SEA） is potent T-cell stimulators which will produce significant tumor inhibition on bladder cancer. This study was to investigate the anti-tumor effect of monoclonal antibody targeted superantigen SEA on human bladder cancer in vivo. Methods： Hu-PBL-SCID mice of human bladder carcinoma, which were transplanted with human peripheral blood lymphocyte intraperitoneally and injected with human bladder carcinoma cells BIU87 subcutaneously, were divided randomly into 3 groups： control, SEA and monoclonal antibody targeted SEA group, subject to subcutaneous injection with PBS, SEA, or monoclonal antibody targeted SEA surrounding the xenografi respectively. Tumor size and blood flow were monitored under the SonoSite ultrasound system. Results： Compared with control and SEA groups, the tumor size and blood flow of monoclonal antibody targeted SEA group were significantly depressed （P〈0.05）. Conclusion： Monoctonal antibody targeted SEA significantly depresses the xenograft growth, possibly through the down-regulation of tumor blood flow.