脱落酸对人胰腺癌细胞增殖的影响

Effects of abscisic acid on proliferation of human pancreatic cancer cells

目的探讨脱落酸（ABA）对人胰腺癌细胞株（PANC-1）增殖的影响及其作用机制。方法细胞计数试剂盒（CCK-8）法筛选出ABA处理浓度，实验分为对照组和ABA处理组，处理组设3个处理浓度，0．001、0．010、0．100mmol／L。通过细胞形态观察、CCK-8法及膜联蛋白V／碘化丙锭（AnnexinV／PI）方法观察ABA对PANC-1增殖的影响。逆转录一聚合酶链反应（RT-PCR）及Western blot法分别检测端粒酶mRNA（hTERTmRNA）及半胱氨酰天冬氨酸特异性蛋白酶（Caspase）-3、B淋巴细胞／白血病12（bcl-2）、细胞周期素（Cyclin）D1蛋白的表达。结果ABA使细胞增殖减慢（24h：F=52．253，P〈0．01；48h：F=107．461，P〈0．01），促进凋亡（对照组和各处理组抑制率分别为4．26％、15．42％、22．02％、39．95％）。处理组hTERTmRNA表达下降，Caspase-3表达增强，bcl-2及Cyclin D1表达减弱，此效应随ABA浓度升高而明显。结论ABA能抑制PANC-1细胞增殖，促进凋亡，其机制是活化Caspase-3，降低hTERTmRNA及bcl-2、Cyclin D1蛋白的表达。

Objective To probe the effects of abscisic acid （ABA） on proliferation of human pancreatic cancer cell line （PANC-1） and the mechanisms. Methods The processing concentration of ABA was screened out by cell counting kit-8 （CCK-8） assay. The experiment was divided into control group and ABA-treated groups （0. 001,0.01, 0. 1 mmol/L）. The effects of ABA on proliferation of PANC-1 cells were observed by using morphologic examination, CCK-8 assay and Annexin V/PI staining. The expression of hTERT mRNA was detected by using reverse transcription-polymerase chain reaction （RT-PCR）. The expressions of Caspase-3, B lymphocytes/leukemia-2 （bcl-2） and Cyclin D1 was detected by using Western blotting. Results ABA slowed down proliferation of PANC-1 cells （24 h： F = 52. 253, P 〈 0. 01 ; 48 h ： F= 107.461 ,P 〈0. 01 ） as compared with control group. ABA could promote apoptosis of PANC-1 cells （ The inhibitory rate in control group and ABA groups was respectively 4. 26%, 15.42%, 22. 02%, and 39.95% ）. The expression of hTERT mRNA was decreased, the expression of Caspase-3 was increased, while bcl-2 and Cyclin D1 were decreased in ABA groups. The effects were concentration-dependent. Conclusion ABA can inhibit proliferation and promote apoptosis of PANC-1 cells probably by activating Caspase-3, and reducing the expression of hTERT mRNA, bcl-2 and Cyclin D1.