红花对肝星状细胞间质胶原酶及Ⅰ型胶原基因表达的影响

Effects of carthamus tinctorius on gene expression of interstitial collagenase and typeⅠ collagen in HSC-T6 cells

目的观察红花对HSC－T6细胞间质胶原酶及Ⅰ型胶原基因表达的影响。方法以不同浓度的红花（1．0、0．5g／L）作用于培养的肝星状细胞株HSC．T6细胞48h，收集细胞，提取总RNA；用逆转录定量聚合酶链反应（RT－PCR）方法测定间质胶原酶（MMP13）及Ⅰ型胶原的基因表达水平。结果MMP13基因表达水平在红花（1．0、0．5g／L）两组分别为0．63±0．10、0．40±0．07，而空白对照组为0．26±0．04。Ⅰ型胶原基因表达水平在红花（1．0、0．5g／L）两组分别为1．30±0．20、1．97±0．36，而空白对照组为3．12±0．46。结论红花可明显促进HSC-T6细胞间质胶原酶的基因表达，并明显抑制Ⅰ型胶原的基因表达；且这些作用与剂量有关。

Objective To investigate the effects of carthamus tinctorius on gene expression of interstitial collagenase and type Ⅰ collagen in HSC-T6 cells. Methods Hepatic stellate cells （HSC-T） were cultured in medium containing different concentrations of carthamus tinctorius （1.0, and 0. 5 g/L） and harvested after incubation for 48 h. Total RNA of HSCs was isolated and gene expression levels of interstitial collagenase （MMP13） and type Ⅰcollagen were detected by using reverse transcription polymerase chain reaction. Results MMP13 gene expression levels in different groups of carthamus tinctorius （ 1.0, and 0. 5 g/L） were 0. 63 ±0. 10 and 0. 40 ±0. 07 respectively, and that in control group was 0. 26 ±0. 04. Type Ⅰ collagen gene expression levels in different groups of earthamus tinctorius （ 1.0, and 0. 5 g/L） were 1.30 ±0. 20 and 1.97 ±0. 36 respectively, and that in control group was 3.12 ±0.46. Conclusion Carthamus tinctorius may significantly enhance MMP13 gene expression in HSC-T6 ceils, but inhibit type Ⅰ collagen gene expression remarkably in a dose-dependent manner.