红花对肝星状细胞c-fos和c-jun基因表达的调节

Inhibitory effects of carthamus tinctorius on c-fos and c-jun gene expression in hepatic stellate cells

目的观察红花对肝星状细胞c-fos和c-jun基因表达的影响。方法在培养的肝星状细胞株中分别加入不同浓度的红花（终质量浓度分别为1．0、0．5g／L）；于8、24、48、72h4个时间点分别收集细胞，提取肝星状细胞总RNA；用逆转录．聚合酶链反应（RT-PCR）方法测定c-fos和c-jun的基因表达水平。结果红花两组HSC－T细胞c－fos基因表达水平在8h（0．49±0．13、0．34±0．11）、24h（0．42±0．10、0．26±0．08）、48h（O．31±0．07、0．20±0．04）、72h（0．23±0．05、0．12±0．04）4个时间点均明显低于对照组（0．63-t-O．13、0．50士0．12、0．43±0．06、0．32±0．04）；c-jun基因表达水平在8h（0．70±0．14、0．46±0．11）、24h（0．57±0．10、0．43±0．07）、48h（0．42±0．09、0．26±0．06）、72h（0．31±0．05、0．12±0．04）4个时间点也均明显低于对照组（0．93±0．13、0．80-4-0．12、0．63±O．10、0．46±0．07）；随剂量加大，HSC-T细胞c-fos、c-jun基因表达水平逐渐降低；两组间差异有统计学意义（P〈0．05）。结论红花对肝星状细胞c－fos和c-jun基因表达有明显下调作用。

Objective To investigate the effects of carthamus tinctorius on c-fos and c-jun gene expression in hepatic stellate cells （HSCs）. Methods Hepatic stellate cell＇ line HSC-T6 cells were cultured in medium containing different concentrations of carthamus tinctorius （ 1.0, and 0. 5 g/L） and harvested at differeni incubation periods （8, 24, 48, and 72 h）. Total RNA of HSCs was isolated, and c-fos and c-jun gene expression levels were detected by using reverse transcription polymerase chain reaction. Results C-fos gene expression levels of HSCs in two carthamus tinctorius-treated groups were significantly lower than in control group （0. 63 ± 0. 13, 0. 50 ± 0. 12, 0.43 ± 0.06, 0. 32 ± 0. 04 ） after exposure to carthamus tinctorius for 8 h （0.49 ± 0. 13, 0. 34 ± 0. 11 ）, 24 h （0.42 ± 0. 10, 0. 26 ± 0.08）, 48 h （0. 31 ± 0. 07, 0. 20 ± 0.04） , and 72 h （0. 23 ± 0.05, 0. 12 ± 0. 04 ）. C-jun gene expression levels of HSCs in two carthamus tinctorius-treated groups were also significantly lower than in control group （0. 93 ± 0. 13,0. 80 ± 0. 12, 0. 63 ± 0. 10, 0. 46 ± 0.07 ） after exposure to carthamus tinctorius at 8 h （ 0. 70 ± 0-14,0.46±0.11）, 24h （0.57±0.10, 0.43±0.07）, 48h （0.42±0.09, 0.26±0.06）, and 72h （0. 31 ±0. 05, 0. 12 ±0. 04）. The gene express＇ion levels of c-fos and c-jun in HSCs were decreased gradually as the dosage of carthamus tinctorius increased, and there were significant difference in the c-los, and c-jun gene expression between two carthamus tinctorius-treated groups. Conclusion Carthamus tinctorius may down-remalate c-fos and c-jun gene exoression in HSCs.