经典瞬时受体电位通道蛋白1在致石豚鼠胆囊平滑肌中的表达

The expression of ransient receptor potential canonical 1 in the smooth muscle of guinea pig gall- bladder and its role in stone formation

目的观察豚鼠胆囊结石形成过程中胆囊平滑肌经典瞬时受体电位通道蛋白1（TRPC1）的表达变化及其对胆囊平滑肌收缩功能的影响。方法20只雄性豚鼠随机均分为对照组和实验组，对照组给予普通饲料喂养，实验组给予致石饲料喂养。喂养60d后剖杀，观察豚鼠胆囊结石形成，检测胆囊体积（FV）j空腹胆囊胆汁量（FB）和胆汁胆固醇含量（TC），并用实时定量聚合酶链反应（Real-time PCR）检测胆囊平滑肌组织TRPCImRNA的表达量。结果实验组有7只豚鼠观察到有胆泥或胆囊结石形成，对照组胆囊未见胆泥或结石形成；实验组FV[（1．78±0．22）cm3]、FB[（1．39±0．16）m1]、TC[（0．05±0．01）mmol／L]水平均高于对照组（P〈0．05）；实验组TRPC1 mRNA（0．7057±0．0627）在胆囊平滑肌的表达量较对照组显著下降（P〈0．05）。结论高胆固醇饮食致石过程中胆囊平滑肌中TRPCI的表达降低，从而可能导致胆囊平滑肌收缩功能减弱，胆囊排空能力下降，使胆囊结石易于形成。

Objective To assess the expression of ransient receptor potential canonical 1（ TRPC1 ） in gallbladder smooth muscle during the process of stone formation and its effects on contractile function of gallbladder smooth muscle. Methods ： 20 male guinea pigs were randomly divided into normal diet group（ control group）, high-cholesterol diet group（ experiment group）, each group has 10 guinea pigs. Both groups were fed for 60 days. Killing them and get the gallbladder to calculate the fasting gallbladder volume （FV） , and measure the fasting gallbladder bile （FB） , and collect some bile to test the content of cholesterol （TC）. The biliary sludge or gallstone formation was observed. Real-time quantitative polymerase chain reaction （Real-time PCR） was adopted to detect TRPC1 mRNA in gallbladder smooth muscle. Results 7 guinea pigs in experiment group have formed biliary sludge or gallstone, no sludge or gallstone was formed in contro]e group. The FV [（1.78±0.22） cm3], FB [（1.39±0.16） ml], TC [（0.05± 0. 01 ） mmol/L] levels in gallbladder bile of experiment group were higher than control group （.P 〈 0. 05 ）. The expression of TRPC1 mRNA （0. 7057 ±0. 0627 ） in gallbladder tissues of experiment group was lower than control group （P 〈 0. 05 ）. Conclusion TRPC1 mRNA in gallbladder decreases during the process of gallstone formation by high-cholesterol diet, which might reduce the contractile function of gallbladder smooth muscle and the ability of gallblader emptying. And it may facilitate the formation of gallstone.