微电场网对肺癌细胞PC9和A549细胞膜通透性的影响

Effects of microelectrifield net on non-small cell lung cancer PC9 and A549 cell lines

目的观察不同参数下微电场网对人非小细胞肺癌细胞株PC9和A549的影响。方法利用微电场网发生仪对细胞株PC9和A549在10、25、40V／cm电场强度下，作用1h和2h，观察PC9和A549细胞的增殖、凋亡和细胞膜通透性改变。结果电场强度40V／cm、作用时间1h，PC9的增殖抑制率和凋亡率分别为62．1％和27．5％，A549的增殖抑制率和凋亡率分别为55．0％和26．4％（P〈0．05）。电场强度25V／cm、作用时间1h，PC9的增殖抑制率和凋亡率分别为31．9％和14．1％，A549的增殖抑制率和凋亡率分别为30．5％和13．8％（P〈0．05）。电场强度10V／cm，作用1h和2h，微电场网对PC9、A549细胞株均无明显抑制作用。此时，微电场网组细胞的荧光染色阳性率明显高于对照组，PC9细胞荧光染色阳性率为（15．4±2．9）％，A549细胞荧光染色阳性率为（18．1±2．1）％。结论当电场强度为10V／cm时，作用时间2h以内，微电场网增加PC9和A549细胞膜通透性是安全有效的。

Objective To observer the effects of microelectrifield net （MEFN） with different parameters on non-small cell lung cancer PC9 and A549 cell lines. Methods PC9 and A549 cell lines were used to assess how voltage and duration of MEFN affected the cells. Cell growth inhibition rate, apoptosis and membrane permeability were observed. Results When electric intensity was 40 V/cm （ duration 1 h）, growth inhibition rate and cell apoptosis rate of PC9 cells were 62. 1% and 27.5%, and those of of A549 cells were 55.0% and 26.4% respectively. When electric intensity was 25 V/cm （duration 1 h）, growth inhibition rate and cell apoptosis rate of PC9 ceils were 31.9% and 14. 1%, and those of A549 cells were 30. 5% and 13.8%, respectively. When electric intensity was 10 V/cm （duration 1 h or 2 h）, there was no significant growth inhibition and apoptosis. Under this condition, membrane permeability in MEFN group was higher than in control group, and the immunofluorescence stain positive rate of PC9 and A549 cells was （ 15.4± 2.9） % and （ 18. 1±2. 1 ） % respectively. Conclusion When electric intensity is 10 V/cm and duration less than 2 h, MEFN can increase membrane permeability of PC9 and A549 cells safely and effectively.