载A3NEP1-40脊髓组织工程支架材料控释规律的研究

Controlled-release law of A3NEPI-40 using self-assembly nano-materials of RADA-IKVAV/FRM as carrier

目的探讨A3NEP1-40以RADA-IKVAV／FRM为载体的缓释规律。方法DMEM／F12溶液能触发RADA-IKVAV／FRM自组装，包裹A3NEP1-40形成均质水凝胶。将包裹125μg及25μg A3NEPl40的水凝胶各自放入2．5ml及0．5ml磷酸盐缓冲液（PBS）中进行释放，并依此分为A、B、C．D4组。应用高效液相色谱仪检测并计算A3NEP1-40每天释放量及累计释放量，统计学分析释放规律。结果（1）最初的24h呈爆发释放，40％的A3NEPl-40自凝胶内释出；平稳释放阶段内样本中可持续检测到A3NEP1-40释放达40d。（2）A组与B组、C组与D组差异无统计学意义，A组与C、D两组、B组与C、D两组差异有统计学意义（P〈0．05）。结论RADA-IKVAV／FRM可以控制A3NEP1-40释放速度，达到缓释效果，是一种较为理想的神经组织工程材料，同时也可以用作载体构建缓释药物。

Objective To investigate the controlled-release law of A3NEP1-40 encapsulated in the hydrogel of RADA-IKVAV/FRM formed by self-assembly. Methods DMEM/F12 solution can trigger RADA-IKVAV/FRM seff-assembly into a porous homogeneous hydrogel, and encapsulate A3NEP1-40 into the internal voids of the hydrogel via hydrophobic interaction. The hydrogels containing 125 μg or 25μg A3NEP1-40 were put a PBS buffer to formulate a controlled-release system. Four groups were Created according to the load of A3NEP1-40 and the buffer volume labeled A, B, C, D. The concentration of A3NEP1-40 in the sample was detected by using high performance liquid chromatography, and the release amount of a day and the cumulative release amount were calculated. The SPSS13.0 statistical software was used to analyze the controlled-release law of A3NEP1-40. Results （ 1 ） At the first 24 h, there was a burst release phase, approximately 40% of the loaded A3NEP1-40 released from gel; in the steady release stage, sustainable A3NEP1-40 release could be detected in the sample for 40 days; （2） There was no statistically significant difference in the A3NEP1-40 release between group A and group B, or between group C and group D, but there was significant difference between groups A and C, groups A and D, groups B and C, or groups B and D （ all P 〈 0. 05 ）. Conclusion The release speed of A3NEP1-40 can be controlled by self-assembly nanometer polypeptide gel materials and a sustained release effect can be achieved. Self-assembly nanometer polypeptide hydrogel containing RADA-IKVAV/FRM is a very good controlled-release carrier, and can be applied to nerve tissue engineering to promote spinal cord injury repair.