菌株DM8320安全评价及其发酵乳对大鼠肾结石形成的影响

Safety evaluation of Lactobacillus DM8320 and effects of its fermented milk on rat kidney stone formation

目的对具有降解草酸能力的菌株DM8320进行初步安全性评价并观察其发酵乳对肾结石模型大鼠的影响。方法通过检测DM8320的质粒,细菌易位试验以及急性毒性试验对DM8320进行初步安全性评价。构建大鼠肾结石模型的同时灌胃目的菌株的牛奶发酵乳,实验第29天,收集24 h尿液并检测尿液中钙离子、草酸的含量。取大鼠左肾制作石蜡切片,观察各个实验组结石发生情况。结果菌株DM8320中不含质粒,未发生细菌易位现象,不具有毒性。DM8320发酵乳可降低肾结石模型大鼠尿中的草酸和钙离子含量。观察各组大鼠肾脏石蜡切片,结石组中结石含量最多,低剂量组次之,高剂量组肾结石结晶含量较少。结论菌株DM8320具有一定的安全性,其发酵乳对Wistar大鼠肾结石的形成具有一定的抑制作用。

Objective To assess the safety of Lactobacillus DM8320 preliminarily and observe the effects of its fermented milk on rat kidney stone formation.Methods Acute toxicity test,bacterial translocation test and plasmid detection test were performed.In the model construction process of Wistar rats with kidney stones,fermented milk of DM8320 was administered.On the 29th day,the body weight and the contents of calcium and oxalic acid in urine were detected.Paraffin sections of rat left kidneys were made to observe the stone occurrence of each experimental group.Results DM8320 strains had no plasmids,no acute toxicity,and did not translocate in healthy rats.DM8320 fermented milk had reduced the oxalic acid and calcium contents in the urine of rats with kidney stones.Paraffin section results showed that the calcium oxalate content of the stone group was the largest,followed by the low dose treatment group,and that of the high dose treatment group was the least.Conclusion Strain DM8320 has a certain degree of safety,and its fermented milk can inhibit the formation of kidney stones in Wistar rats.