氯氰碘柳胺与环丙沙星对耐甲氧西林金黄色葡萄球菌的体外联合药敏试验分析

Combination susceptibility testing of methicillin-resistant Staphylococcus aureus to closantel and ciprofloxacin

目的评价氯氰碘柳胺与环丙沙星联用对60株环丙沙星均为耐药的耐甲氧西林金黄色葡萄球菌(MRSA)的体外抗菌效应。方法采用棋盘法设计,微量肉汤稀释法测定不同浓度组合的抗菌药物对60株甲氧西林耐药的金黄色葡萄球菌(MRSA)的最低抑菌浓度,并计算部分抑菌浓度指数(FICI)判定联合效应。结果氯氰碘柳胺与环丙沙星联合用药后,其FICI分布:FIC≤0.5为20.0%,0.5<FIC≤1为66.7%,1<FIC≤2为13.3%,FIC>2为0。结论体外氯氰碘柳胺与环丙沙星联用对60株耐甲氧西林金黄色葡萄球菌的作用主要为相加和协同作用。

Objective To evaluate the antibacterial effect of combined closantel and ciprofloxacin on 60 strains of ciprofloxacin-and methicillin-resistant Staphylococcus aureus.Methods The protocol was designed with checkerboard method and the MICs of closantel combined with ciprofloxacin on 60 methicillin-resistant Staphylococcus aureus were determined by broth dilution method;the FICI（fractional inhibitory concentration index） was calculated and the effects of the combination of the two drugs were determined.Results When treated with the combination of closantel and ciprofloxacin,the distributions of FICI were as follows： 20.0% when FIC ≤ 0.5;66.7% when 0.5 FIC ≤ 1;13.3% when 1 FIC ≤ 2,and 0 when FIC 2.0.Conclusion The in vitro effect of closantel combined with ciprofloxacin on 60 methicillin-resistant Staphylococcus aureus were mainly effect of additivity and synergism.