建立同时测定人血浆中拉莫三嗪和奥卡西平的HPLC法

Establishment of HPLC Method for Simultaneous Determination of Lamotrigine and Oxcarbazepine in Human Plasma

目的:建立同时测定人血浆中拉莫三嗪和奥卡西平的方法。方法:采用高效液相色谱法进行测定。以氯唑沙宗为内标,色谱柱为DikmaC18,流动相为甲醇-水-三氟乙酸(40:60:0.0005),流速为1ml/min,检测波长为240nm。结果:拉莫三嗪、奥卡西平血药浓度均在0.5～50μg/ml范围内线性关系良好(r分别为0.9940、0.9925),提取回收率分别为92.50%～95.33%、88.78%～93.12%。拉莫三嗪、奥卡西平3种质量浓度(25、10、2μg/ml)的5个平行样品连续测定的RSD分别为4.01%、6.15%、4.64%和3.05%、4.27%、9.01%。结论:本方法操作简单、回收率高、精密度好,可以同时用于测定拉莫三嗪和奥卡西平的血药浓度。

OBJECTIVE： To establish the method for simultaneous determination of plasma concentrations of lamotrigine and oxcarbazepine. METHODS： HPLC method was adopted using chlorzoxazone as internal standard. The determination was performed on Dikma Ct8 column with mobile phase consisted of methanol-water-trifluoroacetic acid （40：60：0.0005） at the flow rate of 1 ml/ min. The detection wavelength was 240 nm. RESULTS： The linear ranges of lamotrigine and oxcarbazepine were both 0.5-50 lag/ml （r=0.994 0 and r=0.992 5）; extraction recoveries were 92.50%-95.33% and 88.78%-93.12%. RSD of continuous sampling for 5 parallel samples of three concentrations of lamotrigine and oxcarbazepine （25, 10, 2 μg/ml） were 4.01%, 6.15%, 4.64% and 3.05%, 4.27%, 9.01%, respectively. CONCLUSION： The method is simple, with high recovery and good precision. It is suitable for the determination of lamotrigine and oxcarbazepine concentration in plasma.