摘要
目的:建立同时测定人血浆中拉莫三嗪和奥卡西平的方法。方法:采用高效液相色谱法进行测定。以氯唑沙宗为内标,色谱柱为DikmaC18,流动相为甲醇-水-三氟乙酸(40:60:0.0005),流速为1ml/min,检测波长为240nm。结果:拉莫三嗪、奥卡西平血药浓度均在0.5~50μg/ml范围内线性关系良好(r分别为0.9940、0.9925),提取回收率分别为92.50%~95.33%、88.78%~93.12%。拉莫三嗪、奥卡西平3种质量浓度(25、10、2μg/ml)的5个平行样品连续测定的RSD分别为4.01%、6.15%、4.64%和3.05%、4.27%、9.01%。结论:本方法操作简单、回收率高、精密度好,可以同时用于测定拉莫三嗪和奥卡西平的血药浓度。
OBJECTIVE: To establish the method for simultaneous determination of plasma concentrations of lamotrigine and oxcarbazepine. METHODS: HPLC method was adopted using chlorzoxazone as internal standard. The determination was performed on Dikma Ct8 column with mobile phase consisted of methanol-water-trifluoroacetic acid (40:60:0.0005) at the flow rate of 1 ml/ min. The detection wavelength was 240 nm. RESULTS: The linear ranges of lamotrigine and oxcarbazepine were both 0.5-50 lag/ml (r=0.994 0 and r=0.992 5); extraction recoveries were 92.50%-95.33% and 88.78%-93.12%. RSD of continuous sampling for 5 parallel samples of three concentrations of lamotrigine and oxcarbazepine (25, 10, 2 μg/ml) were 4.01%, 6.15%, 4.64% and 3.05%, 4.27%, 9.01%, respectively. CONCLUSION: The method is simple, with high recovery and good precision. It is suitable for the determination of lamotrigine and oxcarbazepine concentration in plasma.
出处
《中国药房》
CAS
CSCD
2013年第6期526-528,共3页
China Pharmacy