整合素连接激酶在大鼠心脏生长发育过程中的表达及意义

Expression of Integrin-linked Kinase During Normal Cardiac Development of Rats

目的观察整合素连接激酶在大鼠心脏不同生长发育阶段的表达变化,探讨整合素连接激酶与正常心肌细胞增殖能力的关系。方法取胚胎期(孕16天)、出生后1天、3天、7天、2月、1年、1.5年7个时间点的大鼠心脏,采用实时定量PCR和Western blot方法检测心肌组织内整合素连接激酶及反映心肌细胞增殖能力的细胞周期蛋白D1的表达。结果整合素连接激酶在大鼠心脏生长发育的不同阶段均有表达,表现为在胚胎期心肌组织内的表达水平最高,出生后迅速下降并随鼠龄的增加呈递减趋势,至出生后2月趋于稳定。细胞周期蛋白D1的表达变化与整合素连接激酶的表达变化呈相似趋势,相关分析提示整合素连接激酶与细胞周期蛋白D1的表达水平呈正相关。结论在大鼠心脏生长发育过程中伴随有整合素连接激酶的表达下降,这与哺乳动物心肌细胞增殖能力的变化规律相一致。并且整合素连接激酶与细胞周期蛋白D1的表达水平呈正相关,提示整合素连接激酶可能与心肌细胞增殖能力有关。

Aim In order to investigate the relationship between integrin-linked kinase （ILK） and proliferative capacity of cardiac myocytes, we observed the expression of ILK and eyclin D1 in eardiomyoeytes of Sprague-Dawley （SD） rats at different development stages （embryonic, newborn, adult and old）. Methods Hearts of SD rats at different development stages （ fetal day 16, postnatal day 1,3, 7, postnatal month 2, postnatal year 1, 1.5 ） were used to study. Real time polymerase chain reaction （real time PCR） and Western blot analysis were used to assess ILK and cyclin D1 expression. Results The expression of ILK in myocardial tissue decreased progressively with aging in SD rats. Expression of ILK was the highest in the fetal life （ 16 days gestation）, and the level of ILK tended to be stable at 2 month after birth. Expression of cyclin D1 was positively correlated with ILK content. Conclusions The altered ILK and cyclin D1 parallel normal cardiac development, and expression of cyelin D1 is positively correlated with ILK content. ILK may be correlated with proliferative capacity of cardiac myocytes.