急性力竭运动后小鼠肾细胞凋亡水平的变化及牛磺酸的保护作用

Changes of Renal Cell Apoptosis Level after Acute Exhaustive Exercise and Preventive Effect of Taurine in Mice

为探讨急性力竭运动后小鼠(Mus musculus)肾细胞凋亡水平的时相性变化及牛磺酸对肾的保护作用,将56只雄性小鼠随机分为对照组、力竭运动组(分为运动后即刻组、12 h组、24 h组和48 h组)及牛磺酸运动组(分为12 h组和24 h组),每小组8只,一次性力竭游泳运动后检测肾细胞凋亡水平、Bcl-2和Bax蛋白表达、一氧化氮(NO)含量及结构型一氧化氮合酶(cNOS)、诱导型一氧化氮合酶(iNOS)活性的变化。结果显示,力竭运动后各组小鼠肾细胞凋亡水平呈先升高后下降的趋势,其中运动后24 h组的凋亡水平达峰值(P<0.05)。与对照组相比,运动各组Bax表达均显著增强(P<0.05)。除运动后即刻组外,运动各组Bcl-2表达显著减弱(P<0.05)。各组Bax/Bcl-2比值显著升高,并在运动后24 h达峰值(P<0.01),后出现下降趋势。小鼠力竭游泳后24 h和48 h肾组织NO含量显著高于对照组(P<0.05),同时iNOS活性升高(P<0.01),cNOS活性无显著性变化。相比同时刻运动组,牛磺酸运动组小鼠肾细胞凋亡水平、Bax表达及Bax/Bcl-2比值、iNOS活性显著降低(P<0.05),Bcl-2表达显著升高(P<0.05)。以上结果表明,急性力竭运动可导致肾细胞凋亡的发生,iNOS、Bax、Bcl-2水平及Bax/Bcl-2比值可能在肾细胞凋亡的发生过程中发挥重要的介导作用。牛磺酸可通过调控iNOS活性及Bax/Bcl-2比值,抑制急性力竭运动后小鼠肾细胞凋亡的发生。

To study the changes of renal cell apoptosis after acute exhaustive exercise and the protective effect of taurine, forty six male mice （Mus musculus）were randomly divided into control group, exhaustive exercise group （instant group, 12, 24 and 48 hours after exercise group）, and taurine group （12 and 24 hours after exercise group）, 8 mice in each group. Changes of cell apoptosis, Bcl-2 and Bax expressions, nitric oxide （NO） content, constitutive nitric oxide synthase （cNOS） and inducible nitric oxide synthase （iNOS） activity of renal tissue were detected. The results showed that renal cell apoptosis level first increased, and then decreased, with a peak level at 24 h （P 〈 0.05 ）. Compared to the control group, Bax expression in exercise group significantly increased （P 〈 0.05）. Bcl-2 expression of exercise groups significantly decreased except forinstant group （P 〈 0.05 ）. The ratio of Bax/Bcl-2 increased, and reached a peak level at 24 h （P 〈 0. 05）, and then decreased. The NO level of exercise group was significantly higher than that of the control group 24 h and 48 h after exhaustive exercise （P 〈 O. 05 ）, and iNOS activity increased at the same time （ P 〈 O. O1 ）. The activity of cNOS had no significant change. Compared to the exercise group, the apoptosis level, Bax expression, Bax/Bcl-2 ratio and iNOS activity of taurine group decreased at the same time point （ P 〈0. 05） , while Bcl-2 expression increased （P 〈 0. 05）. The results indicate that acute exhaustive exercise leads to renal cell apoptosis, and that iNOS, Bax, Bcl-2 and the Bax/Bcl-2 ratio may play an important role in the process of renal cell apoptosis. Taurine can inhibit renal cell apoptosis by regulating iNOS activity, Bax and Bcl-2 expression as well as the Bax/Bcl-2 ratio.