抑郁症大鼠杏仁核微管相关蛋白表达及神经元凋亡

Expression of microtubule-associated protein and apoptosis in amygdala of depressional rats

目的观察抑郁症大鼠杏仁核磷酸化微管相关蛋白-2(pMAP-2)表达和神经元凋亡的变化,探讨抑郁症的发病机制。方法随机将20只雄性Wistar大鼠分为对照组和模型组,采用慢性不可预见性温和应激(CUMS)方法建立抑郁大鼠模型,采用糖水偏好实验、悬尾实验、Morris水迷宫实验进行行为学检测,免疫印迹方法检测pMAP-2变化,透射电镜观察神经细胞凋亡。结果模型组体重增长率、糖水偏好百分比低于对照组(P<0.05),悬尾不动时间和逃避潜伏期长于对照组(P<0.05);模型组pMAP-2蛋白表达高于对照组(P<0.05);模型组观察到明显的神经细胞凋亡。结论抑郁症大鼠杏仁核神经细胞凋亡增加,可能与MAP-2磷酸化增高有关。

Objective To observe the change of phosphorylated microtubule-associated protein expression and the neuronal apoptosis in amygdala of depression model rats, and study the mechanism of the disease. Methods Twenty male Wistar rats were randomly divided into control group and model group. The depression animal model was produced by giving rats chronic unpredicted mild stress. The depressional behavior was examined by using sucrose preference test, tailsuspension test and Morris water maze. The expression of pMAP-2 protein was detected by using Western blotting and the apoptosis was observed under a transmission electron microscope. Results Change in body weight （%） and preference of sucrose of the model group rats were signifieantly lower, while the tail-suspension immobility and the escape latency time were longer than those of the control group rats （P 〈 0. 05 ）. The expression of pMAP-2 protein of the model group was higher than that of the control group （P 〈 0.05）. Apoptosis increased in amygdala neurons of the model group rats. Conclusion The enhanced neuronal apoptosis in amygdala of depression may be due to the enhanced phosphorylated microtubule-associated protein expression.