流体剪切力与17-β雌二醇对MC3T3-E1细胞增殖活性协同作用的研究

Research of the synergetic action of fluid shear stress and 17-β estradiol on the proliferation of MC3T3-E1 cells

目的探讨对体外培养的成骨细胞增殖活性促进作用最佳的17-β雌二醇的浓度及作用时间、流体剪切力(FSS)的力值及作用时间,以及此双因素共同作用对成骨细胞增殖活性的影响。方法成骨细胞系MC3T3-E1细胞传代培养后,分别对其施加不同浓度的17-β雌二醇和不同力值的FSS,采用MTT法检测细胞的增殖情况,并检测ALP活性,筛选出最佳的浓度及力值;再将二者共同作用于MC3T3-E1细胞,检测其增殖情况和ALP活性。结果浓度为10-8mol.L-1的17-β雌二醇作用5 d时,MC3T3-E1细胞的增殖及ALP活性优于其他17-β雌二醇处理组;FSS力值为12×10-5N作用60 min时细胞的增殖及ALP活性大于其他FSS处理组。当二者同时作用于MC3T3-E1细胞时,细胞的增殖活性大于任一单因素处理组。结论 17-β雌二醇和FSS对成骨细胞活性的促进作用皆有一个适宜的阈值;二者具有协同作用,对成骨细胞分化及增殖功能的影响优于单一因素作用。

Objective To explore the suitable level and action time of 17-β estradiol and fluid shear stress（FSS） and their combined effect on the proliferation of rat osteoblasts in vitro. Methods MC3T3-E1 osteoblasts were adopted after subcultured and different concentrations of 17-β estradiol and FSS values were applied respectively on MC3T3- El, the suitable level of 17-β estradiol and FSS were selected through MTT and alkaline phosphatase（ALP）. Then the two factors at the suitable level were applied simultaneously to MC3T3-E1 to detect the proliferation activity. Results Seventeen-βestradiol （10-s mol·L-1） for 5 d and 12×10^4 N FSS for 60 rain exhibited better effects on the proliferation activity than the other groups respectively, and the combined effect of both factors was better than any single-factor treated group. Conclusion Both 17-[3 estradiol and FSS have a suitable threshold in promoting prolife- ration of osteoblasts, and two-factor treated group exhibits better effect than any other single-factor treated groups. Therefore 17-β estradiol and FSS have a synergetic action on differentiation and proliferation of osteoblasts.