矽肺患者及观察对象血清髓过氧化物酶和对氧磷脂酶-1活力及临床意义

Serum myeloperoxidase activity and serum paraoxonase-1 activity in patients with silicosis and observation subjects and their clinical significance

目的探讨矽肺患者及观察对象血清髓过氧化物酶（myeloperoxidase，MPO）和对氧磷脂酶-1（paraoxonase-1，PON-1）活力及其临床意义。方法选择72例矽肺（Ⅰ期矽肺30例、Ⅱ期22例、Ⅲ期20例）及37例观察对象为病例组，选择110例男性健康体检者为对照组，用双抗体夹心酶联免疫吸附法（EusA）测定血清MPO活力，用化学分光光度法检测血清PON-1活力。结果病例组血清MPO活力为（102．1±15．7）U／L，明显高于健康对照组[（62．4±11．4）U／L]，差异有统计学意义（P〈0．01）.病例组血清PON-1活力为（85．4±15．7）U／ml，明显低于健康对照组[（125．4±13．7）U／ml]，差异有统计学意义（P〈0．01）。Ⅰ、Ⅱ、Ⅲ期矽肺患者的血清MPO活力[（91．3±13．5）、（85．7±14．4）、（88．6±14．5）U／L]明显低于观察对象[（128．4±16．4）U／LI，差异有统计学意义（P〈0．01）。随矽肺期别的增加，血清PON-活力呈下降趋势，Ⅱ、Ⅲ期矽肺患者的PON-1活力[Ⅱ期：（70．4±11．4）U／ml，Ⅲ期：（67．6±13．7）U／m1）]明显低于观察对象[（101．5±14．0）U／ml]和Ⅰ期矽肺[（89．1±10．1）U／m1]，差异有统计学意义（P〈0．01）。结论血清MPO和PON-1对矽肺的早期诊断和病情判断等方面有一定意义。

Objective To investigate the serum myeloperoxidase （MPO） activity and serum paraoxonase-1 （PON-1） activity in patients with silicosis and observation subjects and their clinical significance. Methods Seventy-two patients with silicosis （stage Ⅰ ： 30 cases, stage Ⅱ ： 22 cases, stage Ⅲ ： 20 cases） and 37 observation subjects were selected as a case group, and 110 healthy men were selected as a control group. Serum MPO activity was measured by double-antibody sandwich enzyme-linked immunosorbent assay, and serum PON-1 activity was measured by chemical spectrophotometry. Results Serum MPO activity was significantly higher in the case group than in the control group [（102.1±15.7） U/L vs. （62.4±11.4） U/L, P〈 0.01], but serum PON-1 activity was significantly lower in the case group than in the control group [（85.4±15.7） U/ml vs. （125.4±13.7） U/ml, P〈0.01]. Serum MPO activity was significantly lower in patients with stages Ⅰ, Ⅱ, and Ⅲ silicosis than in the observation subjects [（91.3±13.5） U/L, （85.7±14.4） U/L, and （88.6±14.5） U/L vs. （128.4±16.4） U/L, P〈0.01]. Serum PON-1 activity declined as the stage of silicosis increased; serum PON-1 activity was significantly lower in the patients with stages Ⅱ and Ⅲ silicosis than in the observation subjects and the patients with stage Ⅰ silicosis [（70.4±11.4） U/ml and （67.6±13.7） U/ml vs. （101.5±14.0） U/ml and （89.1±10.1） U/ml, P〈0.01]. Conclusion Serum MPO activity and serum PON-1 activity are valuable for early diagnosis of silicosis and evaluation of patient＇ s condition.