手性Eu(Ⅲ)配合物的合成和作为ct-DNA荧光探针的研究

Synthesis,Characterization of Chiral Eu(Ⅲ) Complexes and Study of Chiral Fluorescence Probe to ct-DNA

通过制备拆分剂氯化(8S,9R)-(-)-N-苄基辛可尼定,拆分了外消旋联萘酚得到其两种对映异构体,以手性联萘酚为原料分别合成了配体(R)-L和(S)-L及其相应的Eu(Ⅲ)配合物(R or S)-L-Eu(NO3)3,并测定了旋光度。分别研究了配合物(R or S)-L-Eu(NO3)3与ct-DNA的作用方式及键合常数,发现配合物与小牛胸腺DNA(ct-DNA)存在插入作用,且(R)-L-Eu3+配合物与ct-DNA的键合能力较强。由于ct-DNA对(R)-L-Eu3+配合物荧光的增敏作用,选择(R)-L-Eu3+配合物作为ct-DNA的手性荧光探针,在最佳实验条件下,其线性范围为0.11×10-5～0.55×10-5 mol·L-1,0.55×10-5～7.8×10-5 mol·L-1,检出限为5.85×10-7 mol·L-1。

Prepared by splitting chloride （8S,9R）- （-）-N-benzyl Keesing Marconiset, resolution of the raeemic binaphthol by its two enantiomers, with chiral binaphthol as raw materials were synthesized （R）-L and （S）-L and Eu （ Ⅲ ） complexes, and their optical ro- tation values were measured. The interaction between ct-DNA and （R or S）-L-Eu（ NO3 ）3 was investigated, the results indicated that intercalation played an important role between ct-DNA and complexes. The binding constant of （R） -L-Eu （ NO3 ） s with ct-DNA was higher than that of （S）-L-Eu（ NO3 ）3. Because ct-DNA could obviously increase the fluorescent intensity of （R）-L-Eu3＋ , （R）-L-Eu （ NO3 ） s was chosen as chiral fluorescence probe to ct-DNA. Under the optimum reaction conditions, the linear range was 0.11 × 10^ -5 -0.55 × 10^ -5 mol.L-1, 0. 55 × 10 ^-3 -7.5× 10 ^-5 mol.L-1 with the detection limit of 5.85 × 10 ^-7 mol.L-1.