反苯环丙胺对结肠癌细胞迁移和侵袭的影响

The effect of tranylcypromine on the invasion and migration of human colon cancer cells

目的探讨抑制赖氨酸特异性脱甲基酶1（1ysine specific demet hylase1，LSDl）活性对人结肠癌细胞迁移的影响。方法本研究应用反苯环内胺抑制LSDl酶的活性，通过划痕试验和Transwall实验在体外观察人结肠癌Lovo细胞迁移和侵袭的变化。用RT—PCR和Western blot法检测LSDl的表达和H3K4me2甲基化水平。结果经过不同剂量反苯环丙胺（40、60和80μmol／L）处理后，细胞增殖实验结果显示对人结肠癌Lovo细胞增殖无影响（P〉0．05）；60μmol／L反苯环丙胺干预后，划痕试验结果发现Lovo细胞迁移明显受抑制。Transwall实验发现穿过小室的细胞数：60μmol／L反苯环丙胺组为（69．7±2．5），而对照组为（222．7±11．0），两者比较差异有统计学意义（P〈0．05）。RT—PCR检测显示Lovo细胞中的LSDlmRNA和蛋白水平的表达无改变，但H3K4me2甲基化水平升高。结论60μmol／L反苯环丙胺能抑制结肠癌Lovo细胞的LSDl酶活性，降低细胞的迁移和侵袭能力。

Objective To investigate the effect of LSD1 on the invasion and migration of human colon cancer cells. Methods MAO inhibitor and gene silence was used to observe the effect of LSD1 on the invasion and migration of human colon cancer Lovo cells by scratch test and Transweil test in vitro. The gene expression of LSD1 was analyzed by RT-PCR and globle histone methylation in Lovo cells was detected by Western blot. Results At concentrations of 40,60 and 80 pμmol/L MAO inhibitor Tranyleypromine （TCP） , have no effect on the cell growth of human colon cancer Lovo cells （P 〉 0. 05 ）. The scratch test showed that of TCP at 60 μmol/L effectively inhibited the migration of Lovo cells. The cell numbers of migration through the well were （ 69. 7± 2. 5 ） （ 60 μmol/L TCP group ） and （ 222. 7 ± 11.0 ） （ control group） （P 〈0. 05） , while 60 Ixmol/L TCP didn＇t affect the expression of mRNA and protein level of LSD1 but increased the expression of H3K4me2 in Lovo cells. Conclusions MAO inhibitor TCP can inhibit the invasion and migration of human colon cancer Lovo cells at a concentration of 60 μmol/L.