1株鸭坦布苏病毒人工感染雏鸭的病理学研究

Pathological Study of Tembusu Virus in Duck by Artificial Infection

7日龄雏鸭经肌肉接种坦布苏病毒BZ株1周后,发病率100%,死亡率为80%。接种鸭全身多数器官出现充血、出血、坏死等症状,病理学变化主要表现在肝实质严重变性、坏死;脾淋巴细胞局部减少;肾小管上皮细胞肿胀;肺淤血,内有大量细胞渗出;胰腺中可见凝固性坏死灶;大脑软脑膜充血、水肿,小脑脑膜上炎性细胞浸润。鸭接种该病毒后,攻毒组的丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、谷氨酰胺转移酶、乳酸脱氢酶水平和血清尿酸的浓度较对照组明显升高。结果表明:坦布苏病毒BZ株可造成全身多器官的病理性损伤以及主要血液生化指标的显著变化,这可能是该病毒致病性的重要机制之一。

One Tembusu virus named BZ was inoculated to 7-day-old ducks. One week later, the incidence rate of 100% and the mortality rate of 80% occurred. Most of duck organs showed the systemic symptoms of congestion, hemorrhage and necrosis. The pathological changes showed severe liver parenchymal degeneration and necrosis; Spleen lymphocytes partial reduced; Renal tubular epithelial cell swelled; Pulmonary congestion, a large number of cells overflowed; Visible coagulation necrosis in the pancreas; Brain congestion and edema, meningeal inflammatory cell of cerebellar infiltrated. Compared with those of control group, the activities of serum alanine amin- otransferase（ALT）, aspartate aminotransferase （ AST）, gamma glutamyltransferase （GGT）, lactic dehydrogenase （LDH） and concentration of uric acid （UA） in virus attacked ducks were obviously increased. The results showed that the pathogenic mechanisms of the virus may be as- sociated with cell necrosis and multiple organ injury and exhaustion.