摘要
目的:观察吡格列酮对大鼠缺血/再灌注损伤心肌转化生长因子β1(TGFβ1)表达的影响。方法:30只SD大鼠随机分为5组(n=6):缺血/再灌注组、吡格列酮5 mg/(kg.d)组、吡格列酮10 mg/(kg.d)组、吡格列酮20 mg/(kg.d)组、吡格列酮20 mg/(kg.d)+过氧化物酶体增殖物激活受体γ(PPARγ)特异性阻断剂GW9662组,利用在体结扎左前降支的方法建立缺血/再灌注损伤模型,脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL法)检测心肌细胞凋亡,RT-PCR方法检测心肌组织TGFβ1 mRNA的变化,Western blot方法检测心肌组织TGFβ1蛋白的变化。结果:TUNEL法显示吡格列酮抑制缺血/再灌注心肌细胞凋亡,吡格列酮上调TGFβ1表达,GW9662逆转吡格列酮对凋亡细胞的抑制作用,抑制吡格列酮促进TGFβ1表达上调的作用。结论:吡格列酮可抑制缺血/再灌注损伤诱导的心肌细胞凋亡,吡格列酮可促进TGFβ1上调,这两种作用是由PPARγ介导的。
Objective： To investigate the effects of pioglitazone on transforming growth factor β1（TGFβ1） expression in ischemia/reperfusion injury myocardium of rats.Methods： Thirty SD rats were randomly divided into five groups（n=6）： ischemia/reperfusion group,pioglitazone 5 mg/（kg·d） group,pioglitazone 10 mg/（kg·d） group,pioglitazone 20 mg/（kg·d） group and pioglitazone 20 mg/（kg·d） ＋ peroxisome proliferator-activated receptor γ（PPARγ） specific antagonist GW9662 group.Left anterior descending coronary artery of rats were ligated for 30 min and reperfused for 120 min to establish the model of ischemia/reperfusion in vivo.RT-PCR was performed to detect the expression of TGFβ1 mRNA.Western blot was performed to detect the expression of TGFβ1 protein.Results： Myocardial apoptosis was significantly suppressed by pioglitazone.Pioglitazone upregulated TGFβ1 expression both in mRNA and protein level.GW9662 reversed the inhibition of myocardial apoptosis and the upregulation of TGFβ1 expression by pioglitazone.Conclusion： Pioglitazone can inhibit the myocardial apoptosis induced by ischemia/reperfusion.Pioglitazone may protect the myocardium from ischemia/reperfusion via upregulation of TGFβ1.This protection may be mediated by PPARγ.
出处
《中国应用生理学杂志》
CAS
CSCD
2013年第1期1-4,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助项目(30270551)
