离体颈动脉窦压力感受器研究方法的改进——窦内压的自动控制

Improved methods for researching isolated carotid sinus baroreceptors automatically controlling for sinus pressure

目的:创建一套离体颈动脉窦压力感受器研究中窦内压力的自动控制系统。方法:制备颈动脉窦-窦神经标本并对其进行灌流。在该系统中,引入一个重要的可接受电脑指令的压力控制装置(PRE-U,Hoerbiger,Deutschland),用以钳制窦内压。通过比较压力指令和相应产生的窦内压来鉴定本压力控制系统的可靠性。结果:利用该系统可以准确实现脉动式、斜坡式、阶跃式等多种窦内压力控制模式,并证实记录到压力依赖性窦神经放电活动。结论:应用这套颈动脉窦内压力控制系统可以实现多种压力模式的控制。该系统为深入研究压力感受器机电换能机制提供了有用且灵活的压力控制方法。

Objective： To develop a system for automatically controlling carotid sinus pressure in the study on baroreceptors.Methods： The preparation containing carotid sinus with parts of the connected vessels and carotid sinus nerve（CS-CSN） were isolated and perfused.A critical pressure controlling component（PRE-U,Hoerbiger,Deutschland） dictated by a computer was integrated into the system to clamp the intrasinus pressure.The pressure command and the relevant intrasinus pressure were compared to evaluate the validity of the pressure controlling system.Results： A variety of sinus pressure-controlling patterns,including pulsation,ramp and step pressures,could be achieved accurately by using the system,and the pressure-dependent discharge activities of sinus nerve were confirmed.Conclusion： This system for clamping carotid sinus pressure could realize multiple pressure-controlling patterns and is a useful and flexible pressure controlling method that could applied in the study on mechano-electric transduction of baroreceptors.