肢体缺血预处理对大鼠海马CA3区和齿状回区p38 MAPK和HSP 70表达的影响

Effect of limb ischemic preconditioning on the expression of p38 MAPK and HSP 70 in CA3 and DG regions of the hippocampus of rats

目的:观察肢体缺血预处理(LIP)后大鼠海马CA3区和齿状回区(DG)p38MAPK和HSP70的表达,以进一步探讨CA3区和DG区的缺血耐受机制。方法:96只Wistar大鼠随机分为sham组和LIP组,LIP组进一步分为LIP6 h、LIP 12h、LIP1 d、LIP2d、LIP3 d、LIP4d和LIP5 d亚组。方法:应用免疫组织化学和Western blot方法观察大鼠海马CA3区及DG区p38MAPK和HSP70的表达。结果:免疫组化及Western blot结果显示,与sham组相比,LIP后大鼠海马CA3区及DG区p-p38MAPK及HSP70的表达呈现出动态的变化,其中p-p38MAPK的表达于LIP后1 d明显增加、3 d达到高峰,LIP后4 d逐渐下降;HSP 70的表达则于LIP后2 d明显增加、3 d达到高峰,LIP后4 d逐渐下降。结论:LIP上调了大鼠海马CA3区和DG区p38MAPK和HSP70的表达。

Objective： To observe the expression of p38 MAPK and HSP 70 in CA3 and dentate gyrus（DG） regions of the hippocampus of rats induced by limb ischemic preconditioning（LIP）.Methods： Ninety-six rats were randomly divided into sham and LIP groups.And the animals in the LIP group were further divided into LIP 6 h,LIP 12 h,LIP 1 d,LIP 2 d,LIP 3 d,LIP 4 d and LIP 5 d subgroups according to the time of reperfusion after LIP.Immunohistochemical staining and Western blot were used to observe the expression of p38 MAPK and HSP 70 in CA3 and DG regions of the hippocampus.Results： The results of the immunohistochemical staining and Western blot were consistent,which indicated that there were fluctuation in the p-p38 MAPK and HSP 70 expression in CA3 and DG regions after LIP compared with those of the sham group.The expression of p-p38 MAPK began to be upregulated 1d after LIP and reached its peak at 3 d and lasted for 4 d after LIP.However,the expression of HSP 70 was significantly up-regulated 2 d after LIP compared to the sham group,reached its peak at 3 d and lasted until the 4 d after LIP.Conclusion： LIP up-regulates the expression of p38 MAPK and HSP 70 in the CA3 and DG regions of the hippocampus of rats.