摘要
[目的]通过将编织材料植入动物胫骨骨隧道内,观察4种PET纤维编织材料与骨界面的结合情况,以及植入材料对活体动物是否存在急性或者长期毒性作用,进而探讨此材料未来作为一种人工韧带或肌腱编织材料应用于临床及组织工程的可行性。[方法]①选取健康新西兰大白兔16只,随机分为4组,重建双后肢胫骨隧道,每只动物对应一种材料,分别于术后2、4、8、12周取材做大体观察,髓腔直径测量,组织学观察,对实验数据行重复测量的两因素多水平分析;②健康新西兰大白兔4只,分为两组,实验组2只,对照组2只,重建模型同上,分别在术后14 d、6个月取主要脏器做石蜡切片HE染色,所得实验数据、图片指标行统计学分析。[结果]①大体观察:16只实验动物伤口均愈合良好,4种PET纤维编织材料的骨隧道内部分均无松弛,且肌肉、筋膜将材料的隧道外部分包裹良好;②隧道直径测量:各个骨隧道的隧道直径均无明显变化,无骨组织及软组织侵蚀现象;③组织学观察:PET纤维编织材料可以和骨组织形成新生骨的直接连接,新生骨可长入材料纤维束间,对纤维编织材料的纤维束形成紧密包裹,使得编织材料与骨界面具有较强的结合力;④PET纤维编织材料对实验动物无急性或长期毒性作用。[结论]PET纤维编织材料植入动物骨隧道后,对活体实验动物无急性或者长期的毒性作用,且在不同的时间点,均有骨样组织及新生骨与编织材料的纤维束包裹或者长入纤维束的间隙中形成紧密结合,使得编织材料与骨质形成直接连接,促进材料-骨的结合,增强材料-骨愈合的强度,是一种理想的有望用于临床或者组织工程上的人工韧带或肌腱制作的编织材料。
[ Objective ] By implanting four different types of polyethylene terephthalate fiber woven material in animal tibial tunnel, to observe the integration of PET fiber woven material with bone interface, and if there are any acute toxicity and long - term toxicity in vivo, and to investigate the possibility of transforming them into an artificial ligament material used in clinical and tissue engineering in the future. [ Methods ] (1)Sixteen healthy New Zealand white rabbits were randomly divided into four groups. Tibial tunnels of the posterior limbs were reconstructed to each animal, one animal corresponds one kind of material. Gross observation was made , histologically, the change of bone tunnel diameter were observed at 2, 4, 8, 12 weeks after surgery, respectively. Four healthy New Zealand white rabbits were randomly divided into two groups. HE staining of paraffin sections of major organs were made at 14 days , 6 months after surgery. Statistical analysis was performed. [ Results] (1)General observation: 16 experimental animals wound healed well, without loosening in the bone tunnels of four different types of poly- ethylene terephthalate fiber woven material , the outer parts of the bone tunnels of material were wrapped by muscles and fascia. (2)The tunnel diameter measurement: there was no obvious change in the diameter of each bone tunnel, no bone tissue and soft tissue erosion. (3)Histological: PET fiber woven material and bone tissue could be directly connected by forming new bone. The fiber bundles of fibrous materials could be tightly packed by new bone, with a strong gripping force between them. (4)There were no acute toxic effects and long - term toxicity effects in experimental animals with PET fiber woven material. [ Conclusion ]PET fiber woven material hasn' t acute toxic and long - term toxicity effects in experimental animals, direct connection developed with bone interface via new bone in bone tunnel. The integration of PET with bone interface has been improved and its intensity is increased. PET fiber woven material is an ideal tendon and ligament production material used in clinical and tissue engineering in the future.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2013年第3期280-284,共5页
Orthopedic Journal of China
基金
国家自然科学基金资助项目(编号:81071457)