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恒河猴Tetherin蛋白对马传染性贫血病毒抑制作用的研究 被引量：2

Rhesus Tetherin inhibits equine infectious anemia virus release
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摘要 Tetherin是新发现的具有限制多种病毒从细胞膜释放功能的细胞蛋白。本研究采用RT-PCR技术从恒河猴巨噬细胞中扩增Tetherin基因,将其插入真核表达载体pEF4/myc-His B中构建重组质粒pHF-Tetherin。并将其转染293T细胞,通过western blot和激光共聚焦分析Tetherin在293T细胞中的表达及亚细胞定位。将pHF-Tetherin分别与马传染性贫血病毒(EIAV)或猴免疫缺陷病毒(SIV)假病毒粒子感染性质粒共转染293T细胞,分析Tetherin对EIAV及SIV出芽的限制作用。研究结果表明,pHF-Tetherin转染293T细胞48 h后可以检测到Tetherin的表达并定位于细胞膜,而且该蛋白具有抑制EIAV及SIV从转染细胞中出芽的生物学功能。 Tetherin is an newly identified cellular protein which possesses a inhibition for retroviruse release from cell. To identify the biologic fimction of tetherin, tetherin gene was amplified by RT-PCR, and inserted into eukaryotic expression vector pEF4/myc-His B to construct recombinant plasmid of pHF-Tetherin, tetherin was expressed and localized on the cell membrane by confocal microscopy observation in the pHF-Tetherin transfected 293T cell. Furthermore, pHF-Tetherin were co-transfected with infectious clone of pFD-GP2 for equine infectious anemia virus （EIV） vrius-like particles （VLPs） or pBR293EAEAN for Simian immunodeficiency virus （SIV） VLPs into 293T cell, the results showed tetherin protein was able to significantly block the release of EIAV VLPs or SIV VLPs on cell surface.
作者 谷勤雍 胡哲 尹鑫 张泽力 吴星亮 魏萍 王晓钧
机构地区 东北农业大学动物医学院 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物研究室 东北林业大学野生动物资源学院
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2013年第2期87-90,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 兽医生物技术国家重点实验室基金项目 国家自然科学基金项目(31072113)
关键词 恒河猴Tetherin 马传染性贫血病毒 猴免疫缺陷病毒 病毒出芽 rhesus macaques Tetherin eqine infectious anemia virus siman acquired immunodeficiency virus virus budding
分类号 S852.65 [农业科学—基础兽医学]
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参考文献11

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同被引文献17

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中国预防兽医学报
2013年 第2期

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