2012年猪嵴病毒流行病学调查及3D基因的遗传变异分析

Epidemiological survey of porcine kobuvirus and genetic variation analysis of 3D gene

为了解猪嵴病毒(PKV)的流行及变异情况,本研究根据PKV的3D基因保守区域核苷酸序列设计一对特异性引物,并利用RT-PCR方法对2012年来自24个省市84个猪场的病料样品共计236份进行3D基因的检测。结果显示:在236份病料中,共有63份为PKV阳性,阳性率为36.69%;而在检测的84个猪场中,共有36个猪场显示PKV阳性,猪场阳性率为42.85%。此外,对2012年分离的9个PKV部分3D基因进行测序分析,并与GenBank中登录的人、牛、猪以及鼠等嵴病毒株相关序列进行遗传变异分析。结果表明9个PKV 3D基因与国内外其他PKV株无论是在核苷酸水平上还是在氨基端水平上均具有较高的同源性,表明PKV的3D基因具有较高的保守性,可以作为病原检测的靶基因。

To investigate the prevalence of porcine kobuvirus （PKV） in China, a pair of primers was designed based on the 3D gene conserved region of porcine kobuvirus and 236 specimen samples, collected from 84 farms in 24 provinces and cities in 2012, were detected for PKV by RT-PCR method. The results indicated that PKV the positive rates both in clinical samples and pig farms were 30.73% （63/236） and 42.85% （36/84）, respectively. Furthermore, the 3D genes of 9 PKV were cloned and sequenced from different pig farms in different provinces, the phylogenetic analysis showed that 9 of the PKV 3D genes belonged to the same branch.