类风湿关节炎和骨关节炎活动期赖氨酰氧化酶的比较

Comparison of lysyl oxidase expression between active rheumatoid arthritis and active osteoarthritis

目的比较赖氨酰氧化酶（LOX）在类风湿关节炎（RA）和骨关节炎活动期滑膜和滑液中的表达，探讨在滑膜炎症和软骨破坏过程中LOX的作用。方法用免疫组织化学方法检测14例RA和24例骨关节炎以及20例膝外伤患者的滑膜中LOX的表达情况，用酶联免疫吸附试验（ELISA）法检测14例RA患者和24例骨关节炎患者活动期滑液中LOX的浓度，统计学分析采用t检验。结果在活动期患者膝关节滑膜中，RA组LOX相对表达量与骨关节炎组比较差异无统计学意义（0．012±0．007，0．013±0．011，P〉0．05）；但RA和骨关节炎活动期患者膝关节滑膜中的LOX蛋白表达量均高于对照组（0．003±0．004，P〈0．01）；RA活动期患者膝关节滑液中的LOX浓度高于骨关节炎活动期患者（P〈0．05）。结论LOX在RA和骨关节炎活动期的滑膜和滑液中高表达，可能与RA和骨关节炎活动期的慢性滑膜炎、软骨骨质破坏和重建及其程度有关。

Objective To investigate the role of lysyl oxidase （LOX） in synovitis and cartilage destruction by comparing the expression of LOX in synovial fluid and synovium of active rheumatoid arthritis （RA） and active osteoarthritis （OA）. Methods LOX in the synovium was detected by immunohistochemistry from 14 patients with active RA, 24 patients with active OA and 20 patients with knee injury （the control group）. LOX in the synovial fluid was measured by enzyme linked immunosorbent assay （ELISA） from 14 patients with active RA and 24 patients with active OA. T-test was used for statistical analysis. Results The level of LOX expression in active RA synovium （0.012±0.007） was similar to that in active OA synovium （0.013±0.011, P〉0.05）. But the expression of LOX in synovium of active RA and active OA was significantly higher than that in synovium of the control group （0.003±0.004, P〈0.01 ）. The amount of LOX in the synovial fluid of active RA [（1.9±1.4） Ixg/ml] was significantly higher than that of active OA [（1.0±0.4） p,g/ml, P〈 0.05 ]. Conclusion High expression of LOX in the synovial fluid and synovium of active RA and active OA suggest that LOX may be involved in chronic synovitis and cartilage destruction, and may be related with the extent of synovitis and cartilage destruction.